ArticleScientific reports2019
Selection of reference genes for qPCR normalization in buffalobur (Solanum rostratum Dunal).
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Reference genes validation in tear fluid for RNA analysis in ocular surface disease.Scientific reports · 2025Article
- Selection and validation of reference genes for qRT-PCR normalization in dayflower (Commelina communis) based on the transcriptome profiling.BMC plant biology · 2024Article
- Application of deep learning for real-time detection, localization, and counting of the malignant invasive weed Solanum rostratum Dunal.Frontiers in plant science · 2024Article
- Evaluating and Validating Sunflower Reference Genes for Q-PCR Studies Under High Temperature Condition.Iranian journal of biotechnology · 2023Article
- Article
- Solanum elaeagnifolium and S. rostratum as potential hosts of the tomato brown rugose fruit virus.PloS one · 2023Article
- Evaluation of stable reference genes for qPCR normalization in circadian studies related to lung inflammation and injury in mouse model.Scientific reports · 2022Article
- Selection and validation of reference genes for RT-qPCR-based analyses ofFrontiers in physiology · 2022Article
- Selection and validation of reference genes for quantitative real-time PCR of Quercus mongolica Fisch. ex Ledeb under abiotic stresses.PloS one · 2022Article
- Validation of Reference Genes for Quantitative PCR in Johnsongrass (Plants (Basel, Switzerland) · 2021Article
- Selection and Validation of Reference Genes for Quantitative Real-Time PCR in White Clover (Plants (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Buffalobur (Solanum rostratum Dunal), which belongs to the Solanaceae family, is a worldwide noxious invasive weed and is listed as one of the top 10 alien invasive species in China. It is harmful to humans and livestock because the entire plant is covered with spines containing toxins. Many studies have analysed the gene expression in this weed species under different stress conditions using quantitative real-time PCR (qPCR). However, until now, there has been no report on suitable reference genes in buffalobur. Herein, 14 candidate reference genes were selected and evaluated for their expression stability in buffalobur in different tissues, at different developmental stages, and in response to several stress conditions using the geNorm, NormFinder, BestKeeper and RefFinder statistical algorithms. The results showed that EF1α, ACT and SAND are suitable reference genes across all samples tested. We recommend the normalization of target gene expression under different experimental conditions using these three genes together. Validation of selected reference genes was achieved by assessing the relative expression levels of P5CS and GI. This work identified the appropriate reference genes for transcript normalization in buffalobur, which will be helpful in future genetic studies of this invasive weed.
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