ArticleScientific reports2019
Analysis by RNA-seq of transcriptomic changes elicited by heat shock in Leishmania major.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
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- PPNet: Identifying Functional Association Networks by Phylogenetic Profiling of Prokaryotic Genomes.Microbiology spectrum · 2023Article
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- Stable Episomal TransfectantPathogens (Basel, Switzerland) · 2022Article
- In vivo transcriptional analysis of mice infected with Leishmania major unveils cellular heterogeneity and altered transcriptomic profiling at single-cell resolution.PLoS neglected tropical diseases · 2022Article
- Transcriptome Analysis of Intracellular Amastigotes of ClinicalMicroorganisms · 2022Article
- Finding Correlations Between mRNA and Protein Levels inFrontiers in cellular and infection microbiology · 2022Review
- AParasitology · 2021Article
- Gene Annotation and Transcriptome Delineation on a De Novo Genome Assembly for the ReferenceGenes · 2021Article
- Protein methyltransferase 7 deficiency in Leishmania major increases neutrophil associated pathology in murine model.PLoS neglected tropical diseases · 2021Article
- Heat Shock Proteins as the Druggable Targets in Leishmaniasis: Promises and Perils.Infection and immunity · 2021Review
- Advances in UnderstandingFrontiers in cell and developmental biology · 2021Review
- Molecular signatures of anthroponotic cutaneous leishmaniasis in the lesions of patients infected with Leishmania tropica.Scientific reports · 2020Article
- Dual transcriptome analysis reveals differential gene expression modulation influenced byMicrobial genomics · 2020Article
- Complete Genome Sequencing and Transcriptome Analysis of Nitrogen Metabolism ofFrontiers in microbiology · 2020Article
- Transcriptomic signatures of cold adaptation and heat stress in the winter ant (Prenolepis imparis).PloS one · 2020Article
- Minor temperature shifts do not affect chromosomal ploidy but cause transcriptomic changes in Leishmania braziliensis promastigotes in vitro.Memorias do Instituto Oswaldo Cruz · 2020Article
- Different Transcriptomic Responses After Moderate and Severe Heat Shock in Trypanosoma cruzi Epimastigotes.The Journal of eukaryotic microbiologyArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Besides their medical relevance, Leishmania is an adequate model for studying post-transcriptional mechanisms of gene expression. In this microorganism, mRNA degradation/stabilization mechanisms together with translational control and post-translational modifications of proteins are the major drivers of gene expression. Leishmania parasites develop as promastigotes in sandflies and as amastigotes in mammalians, and during host transmission, the parasite experiences a sudden temperature increase. Here, changes in the transcriptome of Leishmania major promastigotes after a moderate heat shock were analysed by RNA-seq. Several of the up-regulated transcripts code for heat shock proteins, other for proteins previously reported to be amastigote-specific and many for hypothetical proteins. Many of the transcripts experiencing a decrease in their steady-state levels code for transporters, proteins involved in RNA metabolism or translational factors. In addition, putative long noncoding RNAs were identified among the differentially expressed transcripts. Finally, temperature-dependent changes in the selection of the spliced leader addition sites were inferred from the RNA-seq data, and particular cases were further validated by RT-PCR and Northern blotting. This study provides new insights into the post-transcriptional mechanisms by which Leishmania modulate gene expression.
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