ArticleScientific reports2019
On resolving ambiguities in microbial community analysis of partial nitritation anammox reactors.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Ammonia fumigation combined with nitrogen inhibitor reduces root-knot nematode populations and NFrontiers in microbiology · 2026Article
- Quantifying synthetic bacterial community composition with flow cytometry: efficacy in mock communities and challenges in co-cultures.mSystems · 2025Article
- Nimble vs. torpid responders to hydration pulse duration among soil microbes.Communications biology · 2024Article
- Isolation, identification, and significance of salivaryFrontiers in cellular and infection microbiology · 2023Article
- Controlling anammox speciation and biofilm attachment strategy using N-biotransformation intermediates and organic carbon levels.Scientific reports · 2022Article
- Fructooligosaccharides (FOS) differentially modifies theFrontiers in nutrition · 2022Article
- Metagenomic Insights Into Competition Between Denitrification and Dissimilatory Nitrate Reduction to Ammonia Within One-Stage and Two-Stage Partial-Nitritation Anammox Bioreactor Configurations.Frontiers in microbiology · 2022Article
- AnFrontiers in nutrition · 2022Article
- Microbial community and performance of a partial nitritation/anammox sequencing batch reactor treating textile wastewater.Heliyon · 2021Article
- Lost in translation: the quest for Nitrosomonas cluster 7-specific amoA primers and TaqMan probes.Microbial biotechnology · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PCR-based methods have caused a surge for integration of eco-physiological approaches into research on partial nitritation anammox (PNA). However, a lack of rigorous standards for molecular analyses resulted in widespread data misinterpretation and consequently lack of consensus. Data consistency and accuracy strongly depend on the primer selection and data interpretation. An in-silico evaluation of 16S rRNA gene eubacterial primers used in PNA studies from the last ten years unraveled the difficulty of comparing ecological data from different studies due to a variation in the coverage of these primers. Our 16S amplicon sequencing approach, which includes parallel sequencing of six 16S rRNA hypervariable regions, showed that there is no perfect hypervariable region for PNA microbial communities. Using qPCR analysis, we emphasize the significance of primer choice for quantification and caution with data interpretation. We also provide a framework for PCR based analyses that will improve and assist to objectively interpret and compare such results.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.