Evidence map›Paper›PMID 31060608›Full record

ArticleBMC veterinary research2019

Enhancing the one health initiative by using whole genome sequencing to monitor antimicrobial resistance of animal pathogens: Vet-LIRN collaborative project with veterinary diagnostic laboratories in United States and Canada.

Olgica Ceric, Gregory H Tyson, Laura B Goodman, Patrick K Mitchell, Yan Zhang, Melanie Prarat, Jing Cui, Laura Peak, Joy Scaria, Linto Antony and 34 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
3.4field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 54 citations in OpenAlex.

  1. Evaluating veterinaryJournal of clinical microbiology · 2026
    Article
  2. Human Cases of Carbapenemase-Producing Escherichia coli Linked to Spread Between Animals and the Environment in a Veterinary Facility-Massachusetts, USA, 2023.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2026
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  3. Cross-Sector Antimicrobial Resistance and Virulence inAntibiotics (Basel, Switzerland) · 2026
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  4. Article
  5. Antimicrobial Resistance inAntibiotics (Basel, Switzerland) · 2026
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  6. Review
  7. Article
  8. Article
  9. Population and pan-genomic analyses ofApplied and environmental microbiology · 2025
    Article
  10. Novel Antibacterial Approaches and Therapeutic Strategies.Antibiotics (Basel, Switzerland) · 2025
    Review
  11. Observational
  12. Article
  13. Article
  14. Evolutionary genomic analyses of canineApplied and environmental microbiology · 2024
    Article
  15. Genomic characterization of antimicrobial resistance in 61 aquatic bacterial isolates.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024
    Article
  16. The Genetic Landscape of Antimicrobial Resistance Genes inAntibiotics (Basel, Switzerland) · 2024
    Article
  17. Whole-genome sequences ofMicrobiology resource announcements · 2024
    Article
  18. Article
  19. Article
  20. Genomic Characterization ofAntibiotics (Basel, Switzerland) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

44 authors at 20 institutions in 2 countries.

Olgica CericVeterinary Laboratory Investigation and Response Network (Vet-LIRN), Center for Veterinary Medicine, United States Food and Drug Administration, 8401 Muirkirk Rd, Laurel, MD, 20708, USA. Olgica.Ceric@fda.hhs.gov.ORCID http://orcid.org/0000-0002-1082-4105
Gregory H TysonVeterinary Laboratory Investigation and Response Network (Vet-LIRN), Center for Veterinary Medicine, United States Food and Drug Administration, 8401 Muirkirk Rd, Laurel, MD, 20708, USA.
Laura B GoodmanPopulation Medicine & Diagnostic Sciences, Cornell University, Ithaca, New York, USA.
Patrick K MitchellPopulation Medicine & Diagnostic Sciences, Cornell University, Ithaca, New York, USA.
Yan ZhangOhio Department of Agriculture, Ohio Animal Disease Diagnostic Laboratory, Reynoldsburg, OH, USA.
Melanie PraratOhio Department of Agriculture, Ohio Animal Disease Diagnostic Laboratory, Reynoldsburg, OH, USA.
Jing CuiOhio Department of Agriculture, Ohio Animal Disease Diagnostic Laboratory, Reynoldsburg, OH, USA.
Laura PeakSchool of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA.
Joy ScariaVeterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, USA.
Linto AntonyVeterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, USA.
Milton ThomasVeterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, USA.
Sarah M NemserVeterinary Laboratory Investigation and Response Network (Vet-LIRN), Center for Veterinary Medicine, United States Food and Drug Administration, 8401 Muirkirk Rd, Laurel, MD, 20708, USA.
Renee AndersonPopulation Medicine & Diagnostic Sciences, Cornell University, Ithaca, New York, USA.
Anil J ThachilPopulation Medicine & Diagnostic Sciences, Cornell University, Ithaca, New York, USA.
Rebecca J Franklin-GuildPopulation Medicine & Diagnostic Sciences, Cornell University, Ithaca, New York, USA.
Durda SlavicAnimal Health Laboratory, University of Guelph, Guelph, Canada.
Yugendar R BommineniFlorida Department of Agriculture and Consumer Services, Bronson Animal Disease Diagnostic Laboratory, Kissimmee, FL, USA.
Shipra MohanFlorida Department of Agriculture and Consumer Services, Bronson Animal Disease Diagnostic Laboratory, Kissimmee, FL, USA.
Susan SanchezAthens Veterinary Diagnostic Laboratory, Department of Infectious Diseases, College of Veterinary Medicine, The University of Georgia, Athens, GA, USA.
Rebecca WilkesTifton Veterinary Diagnostic and Investigational Laboratory, The University of Georgia, Tifton, GA, USA.
Orhan SahinDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA.
G Kenitra HendrixAnimal Disease Diagnostic Laboratory, Purdue University, West Lafayette, IN, USA.
Brian LubbersVeterinary Diagnostic Laboratory, Kansas State University, Manhattan, KS, USA.
Deborah ReedBreathitt Veterinary Center, Murray State University, Murray, KY, USA.
Tracie JenkinsBreathitt Veterinary Center, Murray State University, Murray, KY, USA.
Alma RoySchool of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA.
Daniel PaulsenSchool of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA.
Rinosh ManiVeterinary Diagnostic Laboratory, Michigan State University, East Lansing, MI, USA.
Karen OlsenVeterinary Diagnostic Laboratory, University of Minnesota, St. Paul, MN, USA.
Lanny PaceVeterinary Research and Diagnostic Lab System, Mississippi State University, Starkville, MS, USA.
Martha PulidoVeterinary Research and Diagnostic Lab System, Mississippi State University, Starkville, MS, USA.
Megan JacobNorth Carolina State University College of Veterinary Medicine, Raleigh, NC, USA.
Brett T WebbVeterinary Diagnostic Laboratory, North Dakota State University, Fargo, ND, USA.
Sarmila DasguptaNew Jersey Department of Agriculture, Animal Health Diagnostic Laboratory, Ewing Township, NJ, USA.
Amar PatilNew Jersey Department of Agriculture, Animal Health Diagnostic Laboratory, Ewing Township, NJ, USA.
Akhilesh RamachandranOklahoma Animal Disease Diagnostic Laboratory, Oklahoma State University, Stillwater, OK, USA.
Deepanker TewariPennsylvania Department of Agriculture, Pennsylvania Veterinary Laboratory, Harrisburg, PA, USA.
Nagaraja ThirumalapuraPennsylvania Department of Agriculture, Pennsylvania Veterinary Laboratory, Harrisburg, PA, USA.
Donna J KellyPennsylvania Animal Diagnostic Laboratory, New Bolton Center, University of Pennsylvania, Kenneth Square, PA, USA.
Shelley C RankinSchool of Veterinary Medicine, The Ryan Veterinary Hospital Clinical Microbiology Laboratory, University of Pennsylvania, Philadelphia, PA, USA.
Sara D LawhonTexas A&M University, College Station, TX, USA.
Jing WuTexas A&M University, College Station, TX, USA.
Claire R BurbickCollege of Veterinary Medicine, Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman, WA, USA.
Renate ReimschuesselVeterinary Laboratory Investigation and Response Network (Vet-LIRN), Center for Veterinary Medicine, United States Food and Drug Administration, 8401 Muirkirk Rd, Laurel, MD, 20708, USA.
Cornell University · USCenter for Veterinary Medicine · USLouisiana State University · USOhio Department of Agriculture · USSouth Dakota State University · USFlorida Department of Agriculture and Consumer Services · USMississippi State University · USMurray State University · USNew Jersey Department of Agriculture · USPennsylvania Department of Agriculture · USTexas A&M University · USUniversity of Georgia · USHospital of the University of Pennsylvania · USIowa State University · USKansas State University · USMichigan State University · USNorth Carolina State University · USNorth Dakota State University · USOklahoma State University · USPurdue University West Lafayette · US

Funding

Adapting molecular food safety tests to alternative matricesU18FD005144 · FDA · CORNELL UNIVERSITY · PI GOODMAN, LAURA BRUNENGRABER · 2014 to 2019
$516k
Identification and sub-typing of food-born bacteria from animal feed ingredients, animal feed & pet foods samples using Matrix-Assisted Laser Desorption & Ionization Time-of-Flight Mass Spectrometry.U18FD005143 · FDA · OHIO STATE DEPARTMENT OF AGRICULTURE · PI ZHANG, YAN · 2014 to 2018
$495k
One Health; Epidemiology of natural and deliberate contaminants (infectious and toxicities) in animals and animal foodU18FD006157 · FDA · UNIVERSITY OF GEORGIA · PI Binu Velayudhan · 2017 to 2026
$434k
NJDA Animal Health Diagnostic Laboratory and FDA CVM VetLIRN Program Co-operative Agreement to Safeguard Animal and Human HealthU18FD006245 · FDA · NEW JERSEY STATE DEPARTMENTR/AGRICULTURE · PI Amar Patil · 2017 to 2026
$272k
Companion Animal and Animal Food Diagnostic Sample Analysis in Support of FDA Vet-LIRN Activities and InvestigationsU18FD005164 · FDA · UNIVERSITY OF PENNSYLVANIA · PI RANKIN, SHELLEY CATHERINE · 2014 to 2018
$124k
Salmonella in pets in GAU18FD004318 · FDA · UNIVERSITY OF GEORGIA · PI SANCHEZ, SUSAN · 2011 to 2012
$120k
One Health; Epidemiology of natural and deliberate contaminants (infectious and tU18FD004623 · FDA · UNIVERSITY OF GEORGIA · PI SALIKI, JEREMIAH T, SANCHEZ, SUSAN · 2012 to 2016
$97k
Capacity for antibiotic susceptibility testing and bacterial sequencing at Cornell AHDCU18FD006567 · FDA · CORNELL UNIVERSITY · PI GOODMAN, LAURA BRUNENGRABER · 2018 to 2018
$24k
FDA HHS U18 FD004318FDA HHS U18 FD004619FDA HHS U18 FD004621FDA HHS U18 FD004623FDA HHS U18 FD004624FDA HHS U18 FD004625FDA HHS U18 FD004626FDA HHS U18 FD004629FDA HHS U18 FD004632FDA HHS U18 FD004636FDA HHS U18 FD004637FDA HHS U18 FD004638FDA HHS U18 FD004847FDA HHS U18 FD004848FDA HHS U18 FD004849FDA HHS U18 FD005010FDA HHS U18 FD005143FDA HHS U18 FD005144FDA HHS U18 FD005162FDA HHS U18 FD005164FDA HHS U18 FD006151FDA HHS U18 FD006155FDA HHS U18 FD006156FDA HHS U18 FD006157FDA HHS U18 FD006158FDA HHS U18 FD006160FDA HHS U18 FD006164FDA HHS U18 FD006165FDA HHS U18 FD006169FDA HHS U18 FD006171FDA HHS U18 FD006172FDA HHS U18 FD006173FDA HHS U18 FD006175FDA HHS U18 FD006180FDA HHS U18 FD006245FDA HHS U18 FD006567U.S. Food and Drug Administration 1U18FD004632 and U18FD006175U.S. Food and Drug Administration 1U18FD006151U.S. Food and Drug Administration U18FD004619 and U18FD006155U.S. Food and Drug Administration U18FD004621 and U18FD006180U.S. Food and Drug Administration U18FD004623 and U18FD006157U.S. Food and Drug Administration U18FD004624 and U18FD006165U.S. Food and Drug Administration U18FD004625 and U81FD006158U.S. Food and Drug Administration U18FD004626 and U18FD006245U.S. Food and Drug Administration U18FD004629 and U18FD006156U.S. Food and Drug Administration U18FD004636 and U18FD006169U.S. Food and Drug Administration U18FD004637 and U18FD006164U.S. Food and Drug Administration U18FD004638 and U18FD006171U.S. Food and Drug Administration U18FD004847U.S. Food and Drug Administration U18FD004848U.S. Food and Drug Administration U18FD004849U.S. Food and Drug Administration U18FD005010U.S. Food and Drug Administration U18FD005143U.S. Food and Drug Administration U18FD005144U.S. Food and Drug Administration U18FD005162U.S. Food and Drug Administration U18FD005164U.S. Food and Drug Administration U18FD006160U.S. Food and Drug Administration U18FD006172U.S. Food and Drug Administration U18FD006173
6 · The paper itself

Abstract

backgroundAntimicrobial resistance (AMR) of bacterial pathogens is an emerging public health threat. This threat extends to pets as it also compromises our ability to treat their infections. Surveillance programs in the United States have traditionally focused on collecting data from food animals, foods, and people. The Veterinary Laboratory Investigation and Response Network (Vet-LIRN), a national network of 45 veterinary diagnostic laboratories, tested the antimicrobial susceptibility of clinically relevant bacterial isolates from animals, with companion animal species represented for the first time in a monitoring program. During 2017, we systematically collected and tested 1968 isolates. To identify genetic determinants associated with AMR and the potential genetic relatedness of animal and human strains, whole genome sequencing (WGS) was performed on 192 isolates: 69 Salmonella enterica (all animal sources), 63 Escherichia coli (dogs), and 60 Staphylococcus pseudintermedius (dogs).

resultsWe found that most Salmonella isolates (46/69, 67%) had no known resistance genes. Several isolates from both food and companion animals, however, showed genetic relatedness to isolates from humans. For pathogenic E. coli, no resistance genes were identified in 60% (38/63) of the isolates. Diverse resistance patterns were observed, and one of the isolates had predicted resistance to fluoroquinolones and cephalosporins, important antibiotics in human and veterinary medicine. For S. pseudintermedius, we observed a bimodal distribution of resistance genes, with some isolates having a diverse array of resistance mechanisms, including the mecA gene (19/60, 32%).

conclusionThe findings from this study highlight the critical importance of veterinary diagnostic laboratory data as part of any national antimicrobial resistance surveillance program. The finding of some highly resistant bacteria from companion animals, and the observation of isolates related to those isolated from humans demonstrates the public health significance of incorporating companion animal data into surveillance systems. Vet-LIRN will continue to build the infrastructure to collect the data necessary to perform surveillance of resistant bacteria as part of fulfilling its mission to advance human and animal health. A One Health approach to AMR surveillance programs is crucial and must include data from humans, animals, and environmental sources to be effective.

Indexed as

One HealthWhole Genome SequencingAnimalsBacteriaBacterial InfectionsCanadaLaboratoriesUnited StatesVeterinary MedicineAntimicrobial resistanceOne healthSurveillanceWhole-genome sequencing

Identifiers

PMID31060608
PMCPMC6501310
OpenAlexW2947450553

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.