ArticleiScience2019
In Vitro Membrane Remodeling by ESCRT is Regulated by Negative Feedback from Membrane Tension.
Article in iScience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Mechanical regulation of extracellular vesicle activity during tumour progression.Nature biomedical engineering · 2025Review
- Glass Microdroplet Generator for Lipid-Based Double Emulsion Production.Micromachines · 2024Article
- Dissecting membrane interfacial cellular processes: an in vitro reconstitution approach.The European physical journal. Special topics · 2024Review
- Biogenesis of extracellular vesicles in protozoan parasites: The ESCRT complex in the trafficking fast lane?PLoS pathogens · 2023Review
- Stepwise remodeling and subcompartment formation in individual vesicles by three ESCRT-III proteins.iScience · 2023Article
- Snf7 spirals sense and alter membrane curvature.Nature communications · 2022Article
- Femtoliter Injection of ESCRT-III Proteins into Adhered Giant Unilamellar Vesicles.Bio-protocol · 2022Article
- Physical mechanisms of ESCRT-III-driven cell division.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Mechanics and regulation of cytokinetic abscission.Frontiers in cell and developmental biology · 2022Review
- A Modular, Dynamic, DNA-Based Platform for Regulating Cargo Distribution and Transport between Lipid Domains.Nano letters · 2021Article
- The ESCRT-III machinery participates in the production of extracellular vesicles and protein export during Plasmodium falciparum infection.PLoS pathogens · 2021Article
- Protein crowding mediates membrane remodeling in upstream ESCRT-induced formation of intraluminal vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The ESCRTs - converging on mechanism.Journal of cell science · 2020Review
- Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.Nature cell biology · 2020Article
- Effect of Additives on the Foam Behavior of Aviation Coolants: Tendency, Stability, and Defoaming.ACS omega · 2020Article
- Endomembrane Tension and Trafficking.Frontiers in cell and developmental biology · 2020Review
- Building bridges between chromosomes: novel insights into the abscission checkpoint.Cellular and molecular life sciences : CMLS · 2019Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Artificial cells can shed new light on the molecular basis for life and hold potential for new chemical technologies. Inspired by how nature dynamically regulates its membrane compartments, we aim to repurpose the endosomal sorting complex required for transport (ESCRT) to generate complex membrane architectures as suitable scaffolds for artificial cells. Purified ESCRT-III components perform topological transformations on giant unilamellar vesicles to create complex "vesicles-within-a-vesicle" architectures resembling the compartmentalization in eukaryotic cells. Thus far, the proposed mechanisms for this activity are based on how assembly and disassembly of ESCRT-III on the membrane drives deformation. Here we demonstrate the existence of a negative feedback mechanism from membrane mechanics that regulates ESCRT-III remodeling activity. Intraluminal vesicle (ILV) formation removes excess membrane area, increasing tension, which in turn suppresses downstream ILV formation. This mechanism for in vitro regulation of ESCRT-III activity may also have important implications for its in vivo functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.