ArticleScience advances2019
Electrospray sample injection for single-particle imaging with x-ray lasers.
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Article in Science advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32citing papers in PubMed
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32 citing papers in PubMed.
- High-throughput in situ single particle X-ray imaging of dehydrating viral capsids.Light, science & applications · 2026Article
- Biological applications at the AQUA beamline of the EuPRAXIA@SPARC_LAB free electron laser.European biophysics journal : EBJ · 2026Article
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Impact of gas background on XFEL single-particle imaging.Scientific reports · 2025Article
- Direct Observation of the Exciton-Polaron in Single CsPbBrACS nano · 2025Article
- Coaxial helium electrospray for single-particle imaging at X-ray free electron lasers.Journal of synchrotron radiation · 2025Article
- Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography.Nature methods · 2025Review
- Aerosol size determination via light scattering of viruses and protein complexes.Communications physics · 2025Article
- Coherent X-ray diffraction imaging of single particles: background impact on 3D reconstruction.Journal of applied crystallography · 2024Article
- High-Performance Molecular Dynamics Simulations for Native Mass Spectrometry of Large Protein Complexes with the Fast Multipole Method.Analytical chemistry · 2024Article
- Enhancing electrospray ionization efficiency for particle transmission through an aerodynamic lens stack.Journal of synchrotron radiation · 2024Article
- Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments.Scientific reports · 2024Article
- Observation of a single protein by ultrafast X-ray diffraction.Light, science & applications · 2024Article
- Advanced manufacturing provides tailor-made solutions for crystallography with x-ray free-electron lasers.Structural dynamics (Melville, N.Y.) · 2024Article
- Water layer and radiation damage effects on the orientation recovery of proteins in single-particle imaging at an X-ray free-electron laser.Scientific reports · 2023Article
- Are the Gas-Phase Structures of Molecular Elephants Enduring or Ephemeral? Results from Time-Dependent, Tandem Ion Mobility.Analytical chemistry · 2023Article
- Expected resolution limits of x-ray free-electron laser single-particle imaging for realistic source and detector properties.Structural dynamics (Melville, N.Y.) · 2022Article
- Pump-probe capabilities at the SPB/SFX instrument of the European XFEL.Journal of synchrotron radiation · 2022Article
- Neurotransmitter uptake of synaptic vesicles studied by X-ray diffraction.European biophysics journal : EBJ · 2022Article
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Authors and funding
39 authors.
Johan BieleckiLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.ORCID 0000-0002-3012-603X
Max F HantkeLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.ORCID 0000-0003-1251-0465
Benedikt J DaurerLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.ORCID 0000-0002-1887-7551
Hemanth K N ReddyLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Dirk HasseLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.ORCID 0000-0001-8710-327X
Daniel S D LarssonLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.ORCID 0000-0001-7683-6419
Laura H GunnLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.ORCID 0000-0003-2072-0884
Martin SvendaLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Anna MunkeLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.ORCID 0000-0002-5510-2245
Jonas A SellbergLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.ORCID 0000-0003-2793-5052
Leonie FlueckigerARC Centre of Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe University, Melbourne, Victoria 3086, Australia.
Alberto PietriniLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.ORCID 0000-0002-1171-6683
Carl NettelbladLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.ORCID 0000-0003-0458-6902
Ida LundholmLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Gunilla CarlssonLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.ORCID 0000-0002-7565-0177
Kenta OkamotoLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Nicusor TimneanuLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.ORCID 0000-0001-7328-0400
Daniel WestphalLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Olena KulykInstitute of Physics, ELI Beamlines, Academy of Sciences of the Czech Republic, Na Slovance 2, CZ-18221 Prague, Czech Republic.
Akifumi HigashiuraInstitute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan.ORCID 0000-0001-8448-9349
Gijs van der SchotLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.ORCID 0000-0002-6128-4196
Ne-Te Duane LohCentre for BioImaging Sciences, Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117557, Singapore.ORCID 0000-0002-8886-510X
Taylor E WysongLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Christoph BostedtChemical Sciences and Engineering Division, Argonne National Laboratory, Argonne, IL 60439, USA.
Tais GorkhoverLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Bianca IwanStanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.ORCID 0000-0003-4352-013X
M Marvin SeibertLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Timur OsipovLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Peter WalterLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Philip HartLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.ORCID 0000-0002-4569-3926
Maximilian BucherChemical Sciences and Engineering Division, Argonne National Laboratory, Argonne, IL 60439, USA.ORCID 0000-0001-7896-473X
Anatoli UlmerInstitut für Optik und Atomare Physik, Technische Universität Berlin, 10623 Berlin, Germany.
Dipanwita RayLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Gabriella CariniLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Ken R FergusonLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Inger AnderssonLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.ORCID 0000-0002-2729-0787
Jakob AndreassonInstitute of Physics, ELI Beamlines, Academy of Sciences of the Czech Republic, Na Slovance 2, CZ-18221 Prague, Czech Republic.ORCID 0000-0002-3202-2330
Janos HajduLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.ORCID 0000-0002-3747-2760
Filipe R N C MaiaLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.ORCID 0000-0002-2141-438X
Funding
Wellcome Trust
Abstract
The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution.
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