ArticleJournal of reproduction & infertility
Evaluation of Sperm Chromatin Integrity Using Aniline Blue and Toluidine Blue Staining in Infertile and Normozoospermic Men.
Article in Journal of reproduction & infertility. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Article
- Large-scale transcriptomic analyses reveal downstream target genes of ZFY1 and ZFY2 transcription factors in male germ cells.Cell death and differentiation · 2026Article
- Sperm preparation techniques affect biological parameters, fertility indices, and DNA fragmentation in patients with oligo-astheno-teratozoospermia.Reproduction & fertility · 2025Article
- Therapeutic effects of curcumin nanoemulsion on cyclophosphamide-induced testicular toxicity in adult male mice.Clinical and experimental reproductive medicine · 2025Article
- The Study ofInternational journal of fertility & sterility · 2025Article
- Effect of zinc on sperm recovered by swim-up.Journal of assisted reproduction and genetics · 2025Article
- Reduction of post-thawed sperm quality of Kacang goat using the addition of epigallocatechin-3-gallate into the skim milk-egg yolk extender.Open veterinary journal · 2025Article
- Review
- Can Microfluidics Improve Sperm Quality? A Prospective Functional Study.Biomedicines · 2024Article
- Sperm Chromatin Status and DNA Fragmentation in Mouse Species with Divergent Mating Systems.International journal of molecular sciences · 2023Article
- Article
- Male obesity is associated with sperm telomere shortening and aberrant mRNA expression of autophagy-related genes.Basic and clinical andrology · 2023Article
- Possible Harmful Effects of Smoking Hookah on Sperm DNA Fragmentation Index and Protamine Genes Expression in Normozoospermic Men.Substance abuse : research and treatment · 2023Article
- Male factor testing in recurrent pregnancy loss cases: A narrative review.International journal of reproductive biomedicine · 2022Review
- Relationship between sperm quality and total fertilization failure in intracytoplasmic sperm injection and in vitro fertilization cycles: A cross-sectional study.International journal of reproductive biomedicine · 2022Article
- Does a Very Short Length of Abstinence Improve Assisted Reproductive Technique Outcomes in Infertile Patients with Severe Oligo-Asthenozoospermia?Journal of clinical medicine · 2021Article
- Seminal Plasma Protein N-Glycan Peaks Are Potential Predictors of Semen Pathology and Sperm Chromatin Maturity in Men.Life (Basel, Switzerland) · 2021Article
- Idiopathic Infertility as a Feature of Genome Instability.Life (Basel, Switzerland) · 2021Review
- The effects of sesame oil and different doses of estradiol on testicular structure, sperm parameters, and chromatin integrity in old mice.Clinical and experimental reproductive medicine · 2021Article
- Protective effects of curcumin on chromatin quality, sperm parameters, and apoptosis following testicular torsion-detorsion in mice.Clinical and experimental reproductive medicine · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMale infertility is defined as a man lost his ability to fertilize a fertile female naturally. Diagnosis of male infertility cannot be made just according to basic semen analysis. It is necessity to have specific tests for evaluation of chromatin integrity. In this study, an attempt was made to evaluate the sperm chromatin quality in fertile men and infertile subgroup.
methodsAmong 1386 couples, 342 men were categorized into normospermia and 1044 were infertile and they were referred to Yazd Research and Clinical Center for infertility treatment. Standard semen analysis and sperm nuclear maturity tests including aniline blue (AB) and toluidine blue (TB) staining were done. Data were analyzed by SPSS software. The p≤0.05 was considered statistically significant.
resultsThe mean value of TB staining was significantly higher in infertile group compared to normospermic group (p=0.005). Mean of sperm normal morphology was lower in idiopathic infertile men in comparison with normozoospermic men (p= 0.001). The highest negative correlation was obtained between sperm count and AB staining. Progressive motility was negatively correlated with AB and TB staining in both groups but there was no significant difference between AB staining and progressive motility in men normospermia group.
conclusionSperm chromatin staining using AB and TB showed a negative association between sperm chromatin condensation with sperm count, normal morphology and progressive motility. It seems that the AB and TB test may be useful for the assessment of male fertility potential.
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Identifiers
31058054PMC6486564What OpenQuestion holds
Registered trials
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