ArticleMicrosystems & nanoengineering2017
Large-scale microfluidic gradient arrays reveal axon guidance behaviors in hippocampal neurons.
Article in Microsystems & nanoengineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Flow-Induced Shear Stress Combined with Microtopography Inhibits the Differentiation of Neuro-2a Cells.Micromachines · 2025Article
- Information bounds on the accuracy of cell polarization.PloS one · 2025Article
- Biomaterials for neuroengineering: applications and challenges.Regenerative biomaterials · 2025Review
- [Inertial label-free sorting and chemotaxis of polymorphonuclear neutrophil in sepsis patients based on microfluidic technology].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2023Article
- Microfluidic Systems for Neural Cell Studies.Bioengineering (Basel, Switzerland) · 2023Review
- Functional bioengineered models of the central nervous system.Nature reviews bioengineering · 2023Review
- Simplified Cell Magnetic Isolation Assisted SCMicromachines · 2022Article
- Generation of Dynamic Concentration Profile Using A Microfluidic Device Integrating Pneumatic Microvalves.Biosensors · 2022Article
- Microfluidics for Neuronal Cell and Circuit Engineering.Chemical reviews · 2022Review
- A simple, low cost and reusable microfluidic gradient strategy and its application in modeling cancer invasion.Scientific reports · 2021Article
- Comprehensive Evaluation of Stable Neuronal Cell Adhesion and Culture on One-Step Modified Polydimethylsiloxane Using Functionalized Pluronic.ACS omega · 2020Article
- VEGF Upregulates EGFR Expression to Stimulate Chemotactic Behaviors in the rMC-1 Model of Müller Glia.Brain sciences · 2020Article
- BCNNM: A Framework forFrontiers in computational neuroscience · 2020Article
- "Chip-on-a-Transwell" Devices for User-Friendly Control of the Microenvironment of Cultured Cells.ACS applied bio materials · 2019Article
- Sustained Neurotrophin Release from Protein Nanoparticles Mediated by Matrix Metalloproteinases Induces the Alignment and Differentiation of Nerve Cells.Biomolecules · 2019Article
- High-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients.Nature communications · 2018Article
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2 authors.
Funding
Abstract
High-throughput quantitative approaches to study axon growth behaviors have remained a challenge. We have developed a 1024-chamber microfluidic gradient generator array that enables large-scale investigations of axon guidance and growth dynamics from individual primary mammalian neurons, which are exposed to gradients of diffusible molecules. Our microfluidic method (a) generates statistically rich data sets, (b) produces a stable, reproducible gradient with negligible shear stresses on the culture surface, (c) is amenable to the long-term culture of primary neurons without any unconventional protocol, and (d) eliminates the confounding influence of cell-secreted factors. Using this platform, we demonstrate that hippocampal axon guidance in response to a netrin-1 gradient is concentration-dependent-attractive at higher concentrations and repulsive at lower concentrations. We also show that the turning of the growth cone depends on the angle of incidence of the gradient. Our study highlights the potential of microfluidic devices in producing large amounts of data from morphogen and chemokine gradients that play essential roles not only in axonal navigation but also in stem cell differentiation, cell migration, and immune response.
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