ArticleAvicenna journal of medical biotechnology
Optimization of Fermentation Conditions for Reteplase Expression by
Article in Avicenna journal of medical biotechnology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Prokaryotic expression, polyclonal antibody production, and application of yak TGF-β2.Animal reproduction · 2025Article
- Optimizing recombinant mini proinsulin production via response surface method and microbioreactor screening.PloS one · 2025Article
- Delaying production with prokaryotic inducible expression systems.Microbial cell factories · 2024Review
- Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli.Archives of microbiology · 2024Review
- Enhanced production of a recombinant xylanase (XT6): optimization of production and purification, and scaled-up batch fermentation in a stirred tank bioreactor.Scientific reports · 2023Article
- Factors involved in heterologous expression of proteins in E. coli host.Archives of microbiology · 2023Review
- Rational design of a new variant of Reteplase with optimized physicochemical profile and large-scale production in Escherichia coli.World journal of microbiology & biotechnology · 2022Article
- Media optimization for SHuffle T7 Escherichia coli expressing SUMO-Lispro proinsulin by response surface methodology.BMC biotechnology · 2022Article
- Scale-up fermentation of Escherichia coli for the production of recombinant endoglucanase from Clostridium thermocellum.Scientific reports · 2021Article
- Optimization of fermentation conditions for the production of recombinant feruloyl esterase from3 Biotech · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExpression of heterologous proteins at large scale is often a challenging job due to plasmid instability, accumulation of acetate and oxidative damage in bioreactors. Therefore, it is necessary to optimize parameters influencing cell growth and expression of recombinant protein.
methodsIn the present study, the optimal culture conditions for expression of reteplase by
resultsBased on results of 15 experiments, a response surface quadratic model was developed which was used to explain the relation between production of reteplase and three investigated variables. The high value of "R-Squared" (0.9894) and F-value of 51.99 confirmed the accuracy of this model. According to the developed model, the optimum fermentation conditions for reteplase expression were temperature of 32
conclusionOur results indicate the significant role of culture conditions (
Indexed as
Identifiers
31057718PMC6490408What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.