Evidence map›Paper›PMID 31057522›Full record

ArticleFrontiers in microbiology2019

The Impact of DNA Extraction Methods on Stool Bacterial and Fungal Microbiota Community Recovery.

Kristýna Fiedorová, Matěj Radvanský, Eva Němcová, Hana Grombiříková, Juraj Bosák, Michaela Černochová, Matej Lexa, David Šmajs, Tomáš Freiberger

Registry-linked trialOpen access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07505212 (Exploring Tannin-Probiotic Interactions on Microbial Composition and Inflammatory Outcomes in Obesity), which is not on this map. Cited by 70 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed, 1 pooled it
5.6field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT07505212 naactive not recruitingnot on this mapstarted 2026, after this paper: background citation

Exploring Tannin-Probiotic Interactions on Microbial Composition and Inflammatory Outcomes in Obesity

TypeinterventionalSponsorPrairie View A&M UniversityRan2026 to 2026Enrolled20ConditionsObesity (Disorder)ArmsGallotannin-rich mango, Lactiplantibacillus pentosus (probiotic), No Treatment Added
3 · Its place in the literature

Who cites it

70 citing papers in PubMed, 1 synthesis or guideline pooled it, 117 citations in OpenAlex.

  1. Pooled it
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  9. Review
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  11. Photobiomodulation Strengthens Muscles via Its Dual Functions in Gut Microbiota.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
  12. Single-cell quantification of the microbiota by flow cytometry: MicFLY.bioRxiv : the preprint server for biology · 2025
    Article
  13. Article
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10 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Kristýna FiedorováCentre for Cardiovascular Surgery and Transplantation, Brno, Czechia.
Matěj RadvanskýFaculty of Informatics, Masaryk University, Brno, Czechia.
Eva NěmcováCentre for Cardiovascular Surgery and Transplantation, Brno, Czechia.
Hana GrombiříkováCentre for Cardiovascular Surgery and Transplantation, Brno, Czechia.
Juraj BosákDepartment of Biology, Faculty of Medicine, Masaryk University, Brno, Czechia.
Michaela ČernochováCentre for Cardiovascular Surgery and Transplantation, Brno, Czechia.
Matej LexaFaculty of Informatics, Masaryk University, Brno, Czechia.
David ŠmajsDepartment of Biology, Faculty of Medicine, Masaryk University, Brno, Czechia.
Tomáš FreibergerCentre for Cardiovascular Surgery and Transplantation, Brno, Czechia.
Centrum kardiovaskulární a transplantační chirurgie · CZMasaryk University · CZCentral European Institute of Technology – Masaryk University · CZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Our understanding of human gut microbiota in health and disease depends on accurate and reproducible microbial data acquisition. The critical step in this process is to apply an appropriate methodology to extract microbial DNA, since biases introduced during the DNA extraction process may result in inaccurate microbial representation. In this study, we attempted to find a DNA extraction protocol which could be effectively used to analyze both the bacterial and fungal community. We evaluated the effect of five DNA extraction methods (QIAamp DNA Stool Mini Kit, PureLink

Indexed as

16S rDNADNA extraction methodfungal microbiotagut microbiomegut microbiotagut mycobiomegut mycobiotaITS rDNA

Identifiers

PMID31057522
PMCPMC6479168
OpenAlexW2935777223

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.