ArticleJournal of physics D: Applied physics2019
All-optical crosstalk-free manipulation and readout of Chronos-expressing neurons.
Article in Journal of physics D: Applied physics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 17 citations in OpenAlex.
- Active pixel power control for crosstalk-free all-optical neural interrogation.Nature communications · 2026Article
- Multi-Frequency Interpolation X-talk Removal Algorithm: Enabling Combinations of Concurrent Optogenetics and Lock-in Amplification Fiber Photometry via Removal of Optogenetic Stimulation Crosstalk.ACS chemical neuroscience · 2025Article
- Model-Based Explainable Deep Learning for Light-Field Microscopy Imaging.IEEE transactions on image processing : a publication of the IEEE Signal Processing Society · 2024Article
- Large-scale all-optical dissection of motor cortex connectivity shows a segregated organization of mouse forelimb representations.Cell reports · 2022Article
- A kinetic-optimized CoChR variant with enhanced high-frequency spiking fidelity.Biophysical journal · 2022Article
- Comparing synthetic refocusing to deconvolution for the extraction of neuronal calcium transients from light fields.Neurophotonics · 2022Article
- High-performance microbial opsins for spatially and temporally precise perturbations of large neuronal networks.Neuron · 2022Article
- Dynamic real-time subtraction of stray-light and background for multiphoton imaging.Biomedical optics express · 2021Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
All optical neurophysiology allows manipulation and readout of neural network activity with single-cell spatial resolution and millisecond temporal resolution. Neurons can be made to express proteins that actuate transmembrane currents upon light absorption, enabling optical control of membrane potential and action potential signalling. In addition, neurons can be genetically or synthetically labelled with fluorescent reporters of changes in intracellular calcium concentration or membrane potential. Thus, to optically manipulate and readout neural activity in parallel, two spectra are involved: the action spectrum of the actuator, and the absorption spectrum of the fluorescent reporter. Due to overlap in these spectra, previous all-optical neurophysiology paradigms have been hindered by spurious activation of neuronal activity caused by the readout light. Here, we pair the blue-green absorbing optogenetic actuator, Chronos, with a deep red-emitting fluorescent calcium reporter CaSiR-1. We show that cultured Chinese hamster ovary cells transfected with Chronos do not exhibit transmembrane currents when illuminated with wavelengths and intensities suitable for exciting one-photon CaSiR-1 fluorescence. We then demonstrate crosstalk-free, high signal-to-noise ratio CaSiR-1 red fluorescence imaging at 100 frames s
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.