Evidence map›Paper›PMID 31056480›Full record

ArticleStem cell reports2019

Functional Properties of Engineered Heart Slices Incorporating Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Adriana Blazeski, Justin Lowenthal, Renjun Zhu, Jourdan Ewoldt, Kenneth R Boheler, Leslie Tung

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Adriana BlazeskiDepartment of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21205, USA.
Justin LowenthalDepartment of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21205, USA.
Renjun ZhuDepartment of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21205, USA.
Jourdan EwoldtDepartment of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21205, USA.
Kenneth R BohelerDepartment of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21205, USA; Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, Hong Kong, SAR; Division of Cardiology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Leslie TungDepartment of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21205, USA. Electronic address: ltung@jhu.edu.

Funding

Medical Scientist Training ProgramT32GM136577 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI ANDREA L COX · 2020 to 2026
$13.4M
ARVD/C Dysfunction in Human Stem Cell-Derived Cardiac TissueR01HL120959 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI TUNG, LESLIE · 2016 to 2019
$1.9M
JHU Ultima-L Imaging SystemS10RR025544 · NCRR · JOHNS HOPKINS UNIVERSITY · PI TUNG, LESLIE · 2009 to 2009
$158k
NCRR NIH HHS S10 RR025544NHLBI NIH HHS R01 HL120959NIGMS NIH HHS T32 GM136577
6 · The paper itself

Abstract

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) hold great promise for cardiac studies, but their structural and functional immaturity precludes their use as faithful models of adult myocardium. Here we describe engineered heart slices (EHS), preparations of decellularized porcine myocardium repopulated with hiPSC-CMs that exhibit structural and functional improvements over standard culture. EHS exhibited multicellular, aligned bundles of elongated CMs with organized sarcomeres, positive inotropic responses to isoproterenol, anisotropic conduction of action potentials, and electrophysiological functionality for more than 200 days. We developed a new drug assay, GRIDS, that serves as a "fingerprint" of cardiac drug sensitivity for a range of pacing rates and drug concentrations. GRIDS maps characterized differences in drug sensitivity between EHS and monolayers more clearly than changes in action potential durations or conduction velocities. EHS represent a tissue-like model for long-term culture, structural, and functional improvement, and higher fidelity drug response of hiPSC-CMs.

Indexed as

Action PotentialsAnimalsCardiotonic AgentsCell Culture TechniquesCell DifferentiationCells, CulturedElectrophysiological PhenomenaHeartHumansInduced Pluripotent Stem CellsIsoproterenolMyocardiumMyocytes, CardiacSwineTissue EngineeringCardiotonic AgentsIsoproterenolcardiac tissue engineeringelectrophysiologyextracellular matrixinduced pluripotent stem cellsscaffold

Identifiers

PMID31056480
PMCPMC6524004

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.