ArticlePlant signaling & behavior2019
An efficient
Article in Plant signaling & behavior, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed.
- Comparative transcriptome profiling and functional analysis of GmRAMOSA1 as a key regulator of adventitious shoot regeneration in soybean.Plant cell reports · 2026Article
- Hormone-optimized hypocotyl-epicotyl-cotyledonary tri-complex explant system enables efficient soybean transformation with reduced genotype dependence.Plant cell reports · 2026Article
- Environmental dependence of visible anthocyanin accumulation mediated by theFrontiers in plant science · 2026Article
- Functional Analysis of Pheromone Biosynthesis Activating Neuropeptide Receptor Isoforms inCells · 2023Article
- Recalcitrance to transformation, a hindrance for genome editing of legumes.Frontiers in genome editing · 2023Review
- Progress in Soybean Genetic Transformation Over the Last Decade.Frontiers in plant science · 2022Review
- Plant Biosystems Design Research Roadmap 1.0.Biodesign research · 2020Review
- Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic transformation plays a vital role in gene functional study and molecular breeding of soybean. Conventional soybean transformation methods using chemical selectable markers, such as antibiotic or herbicide resistance genes, rely on the identification of positive transgenic lines at advanced developmental stages, making selection procedure labor intensive and time consuming. Utilization of a visual maker to track the transgene would avoid the uncertainty and blindness in the transformation process. In this research, we used green fluorescent protein (GFP) as the selectable marker to detect transgenics at early stages of soybean development. Positive transformants were detected recurrently during each stage of the process based on visualization of the green fluorescence signal, which help us to discard the non-transgenic ones in each stage to reduce the unnecessary experimental cost and lab space. In addition, the positive transgenic seeds can be identified before planting for early detection of transgene and obtain homozygous lines in advance. The method established in this study is also a useful reference for other plant species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.