Evidence map›Paper›PMID 31055774›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2019

Characterization of an OrtT-like toxin of Salmonella enterica serovar Houten.

Luiz Carlos Bertucci Barbosa, Rodolfo Dos Santos Carrijo, Milena Barbosa da Conceição, Jonatas Erick Maimoni Campanella, Edson Crusca Júnior, Thais Oliveira Secches, Maria Celia Bertolini, Reinaldo Marchetto

Erratum issuedOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Luiz Carlos Bertucci BarbosaInstitute of Natural Resources, Federal University of Itajubá, BPS, 1303, Bairro Pinheirinho, Itajubá, MG, 37500-903, Brazil. lcbertucci@gmail.com.
Rodolfo Dos Santos CarrijoInstitute of Chemistry, São Paulo State University, Araraquara, SP, Brazil.
Milena Barbosa da ConceiçãoInstitute of Chemistry, São Paulo State University, Araraquara, SP, Brazil.
Jonatas Erick Maimoni CampanellaInstitute of Chemistry, São Paulo State University, Araraquara, SP, Brazil.
Edson Crusca JúniorInstitute of Chemistry, São Paulo State University, Araraquara, SP, Brazil.
Thais Oliveira SecchesInstitute of Natural Resources, Federal University of Itajubá, BPS, 1303, Bairro Pinheirinho, Itajubá, MG, 37500-903, Brazil.
Maria Celia BertoliniInstitute of Chemistry, São Paulo State University, Araraquara, SP, Brazil.
Reinaldo MarchettoInstitute of Chemistry, São Paulo State University, Araraquara, SP, Brazil.
Universidade Estadual Paulista (Unesp) · BRUniversidade Federal de Itajubá · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Escherichia coli GhoT/GhoS system is a type V toxin-antitoxin system in which the antitoxin GhoS cleaves the GhoT mRNA, controlling its translation. GhoT is a small hydrophobic protein that damages bacterial membranes. OrtT is a GhoT-like toxin, but it apparently lacks a corresponding antitoxin and serves a different physiologic role. Using a profile hidden Markov model approach, a Salmonella enterica serovar Houten genome was screened to obtain homologs of GhoT/OrtT. We only found one protein (referred to here as OrtT-Sal) that shared more sequence identity with OrtT than GhoT. The chromosomal region around the coding sequence of OrtT-Sal suggests that it is an orphan toxin and can be under RpoH activation. To study OrtT-Sal, we chemically synthesized and expressed in E. coli the whole toxin and its N- and C-terminal regions (OrtT-Sal

Indexed as

AntitoxinsBacterial ProteinsBacterial ToxinsGenome, BacterialMolecular StructureSalmonella entericaAntitoxinsBacterial ProteinsBacterial ToxinsGhoTOrphan toxinOrtTToxin–antitoxin system

Identifiers

PMID31055774
PMCPMC6863217
OpenAlexW2943943686

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.