ArticleMolecular biotechnology2019
A Novel Methanol-Free Platform for Extracellular Expression of rhGM-CSF in Pichia pastoris.
Article in Molecular biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 14 citations in OpenAlex.
- Recombinant cytokines produced in Komagataella phaffii: advances in design, production, and purification strategies.Molecular biology reports · 2026Review
- Balancing Doses of EL222 and Light Improves Optogenetic Induction of Protein Production in Komagataella phaffii.Biotechnology and bioengineering · 2025Article
- Methanol-Free Protein Expression in Komagataella phaffii With Magnetic or Non-Magnetic Heating.Microbial biotechnology · 2025Article
- Improvement of RBD-FC Immunogenicity by Using Alum-Sodium Alginate Adjuvant Against SARS-COV-2.Influenza and other respiratory viruses · 2024Article
- An inducible Komagataella phaffii system for protein expression using sorbitol dehydrogenase promoter.Biotechnology letters · 2023Article
- Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris.Microbial cell factories · 2021Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The production of the recombinant proteins under the control of AOX1 promoter is a one of the most common expression systems in the methylotrophic yeast Pichia pastoris which is induced by methanol. The application of this expression platform is restricted by the toxicity and inflammatory nature of methanol, especially in food and pharmaceutical products. Human granulocyte macrophage-colony stimulating factor (hGM-CSF) is an important pharmaceutical protein, playing a crucial role in the proliferation and differentiation of innate immune cells. In this study, a methanol-free expression platform for extracellular expression of hGM-CSF was developed. To attain this goal, a novel constructed expression vector pEP(α)101, carrying the FMD promoter regulating recombinant expression by glycerol derepression was designed. The optimized hGM-CSF gene was subcloned into pEP(α)101 and transformed into P. pastoris. The expression of rhGM-CSF in three different culture media were investigated. Based on the observed heterogeneous glycosylation pattern on SDS-PAGE and western blot, the glycoproteins were deglycosylated to remove carbohydrate units. According to the results, the novel methanol independent P
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