ArticlePlant molecular biology2019
A non-secreted plant defensin AtPDF2.6 conferred cadmium tolerance via its chelation in Arabidopsis.
Article in Plant molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 57 citations in OpenAlex.
- Laminar patterning transcription factors orchestrate spatial metabolite partitioning in Capsicum fruit.Nature plants · 2026Article
- Cadmium toxicity in plants: from transport to tolerance mechanisms.Plant signaling & behavior · 2025Review
- PDF gene family in four Cucurbitaceae species: genome-wide identification, classification and expression in response to biotic stress.BMC plant biology · 2025Article
- Interpopulational Variation in Cyclotide Production in Heavy-Metal-Treated Pseudometallophyte (Plants (Basel, Switzerland) · 2025Article
- Peptides from plants and microorganisms: a defense strategy for biotic and abiotic stresses.Frontiers in plant science · 2025Review
- The Roadmap of Plant Antimicrobial Peptides Under Environmental Stress: From Farm to Bedside.Probiotics and antimicrobial proteins · 2024Review
- Leveraging multi-omics tools to comprehend responses and tolerance mechanisms of heavy metals in crop plants.Functional & integrative genomics · 2024Review
- New Insights into Involvement of Low Molecular Weight Proteins in Complex Defense Mechanisms in Higher Plants.International journal of molecular sciences · 2024Review
- The Uptake, Transfer, and Detoxification of Cadmium in Plants and Its Exogenous Effects.Cells · 2024Review
- Overexpression of vacuolar transporters OsVIT1 and OsVIT2 reduces cadmium accumulation in rice.Plant molecular biology · 2024Article
- Review
- Article
- Comparative Transcriptomics Analysis of Roots and Leaves under Cd Stress inInternational journal of molecular sciences · 2022Article
- Metalloprotein-Specific or Critical Amino Acid Residues: Perspectives on Plant-Precise Detoxification and Recognition Mechanisms under Cadmium Stress.International journal of molecular sciences · 2022Review
- Characterization of an Arabidopsis Defensin-like Gene Conferring Resistance against Nematodes.Plants (Basel, Switzerland) · 2022Article
- Cadmium toxicity in medicinal plants: An overview of the tolerance strategies, biotechnological and omics approaches to alleviate metal stress.Frontiers in plant science · 2022Review
- Article
- Analysis of a gene family for PDF-like peptides from Arabidopsis.Scientific reports · 2021Article
- Advances in "Omics" Approaches for Improving Toxic Metals/Metalloids Tolerance in Plants.Frontiers in plant science · 2021Review
- Genome-scale identification ofPeerJ · 2021Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
key messagePlant defensin AtPDF2.6 is not secreted to the apoplast and localized in cytoplasm. AtPDF2.6 is mainly expressed in root vascular bundles of xylem parenchyma cell, and significantly induced by Cd stress. AtPDF2.6 detoxicate cytoplasmic Cd via chelation, thus enhanced Cd tolerance in Arabidopsis. In order to detoxify the heavy metal cadmium (Cd), plants have evolved several mechanisms, among which chelation represents the major Cd-detoxification mechanism. In this study, we aimed to identify a new defensin protein involved in cytoplasmic Cd detoxification by using plant molecular genetics and physiological methods. The results of bioinformatic analysis showed that the Arabidopsis thaliana defensin gene AtPDF2.6 has a signal peptide that may mediate its secretion to the cell wall. Subcellular localization analysis revealed that AtPDF2.6 is localized to the cytoplasm and is not secreted to the apoplast, whereas histochemical analysis indicated that AtPDF2.6 is mainly expressed in the root xylem parenchyma cells and that its expression is significantly induced by Cd. An in vitro Cd-binding assay revealed that AtPDF2.6 has Cd-chelating activity. Heterologous overexpression of AtPDF2.6 increased Cd tolerance in Escherichia coli and yeast, and AtPDF2.6 overexpression significantly enhanced Cd tolerance in Arabidopsis, whereas functional disruption of AtPDF2.6 decreased Cd tolerance. These data suggest that AtPDF2.6 detoxifies cytoplasmic Cd via chelation and thereby enhances Cd tolerance in Arabidopsis. Our findings accordingly challenge the commonly accepted view of defensins as secreted proteins.
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