ArticleArchives of virology2019
Development of a duplex reverse transcription recombinase-aided amplification assay for respiratory syncytial virus incorporating an internal control.
Article in Archives of virology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Rapid Detection ofMicroorganisms · 2026Article
- Portable, precise, and RNA extraction-free: RT-RAA technology for rapid early RSV identification and prevention.Frontiers in cellular and infection microbiology · 2025Article
- Optimization and Validation of Reverse Transcription Recombinase-Aided Amplification (RT-RAA) for Sorghum Mosaic Virus Detection in Sugarcane.Pathogens (Basel, Switzerland) · 2023Article
- A rapid and highly sensitive multiple detection of human adenovirus type 3, type 7 and respiratory syncytial virus by recombinase-aided reverse transcription PCR.Journal of clinical laboratory analysis · 2023Article
- Advancements in the synergy of isothermal amplification and CRISPR-cas technologies for pathogen detection.Frontiers in bioengineering and biotechnology · 2023Review
- Reverse transcription recombinase-aided amplification assay for H5 subtype avian influenza virus.Virology journal · 2022Article
- Rapid Detection of Multi-Resistance Strains CarryingFrontiers in microbiology · 2022Article
- Development of a RPA-CRISPR-Cas12a Assay for Rapid, Simple, and Sensitive Detection ofFrontiers in microbiology · 2022Article
- Establishment of a reverse transcription recombinase-aided amplification detection method for porcine group a rotavirus.Frontiers in veterinary science · 2022Article
- Recent advances and challenges of biosensing in point-of-care molecular diagnosis.Sensors and actuators. B, Chemical · 2021Review
- Article
- Rapid Detection of Pathogens in Wound Exudate via Nucleic Acid Lateral Flow Immunoassay.Biosensors · 2021Article
- Rapid and Sensitive Detection ofFrontiers in microbiology · 2021Article
- Rapid Detection of New Delhi Metallo-β-Lactamase Gene Using Recombinase-Aided Amplification Directly on Clinical Samples From Children.Frontiers in microbiology · 2021Article
- RAP: A Novel Approach to the Rapid and Highly Sensitive Detection of Respiratory Viruses.Frontiers in bioengineering and biotechnology · 2021Review
- Multiple-centre clinical evaluation of an ultrafast single-tube assay for SARS-CoV-2 RNA.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2020Article
- Trends in MERS-CoV, SARS-CoV, and SARS-CoV-2 (COVID-19) Diagnosis Strategies: A Patent Review.Frontiers in public health · 2020Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
Human respiratory syncytial virus (RSV) is a common viral pathogen that causes lower respiratory tract infections in infants and children globally. In this study, we developed a duplex reverse transcription recombinase-aided amplification (duplex-rtRAA) assay containing an internal control in a single closed tube for the detection of human RSV. The internal control in the amplification effectively eliminated false-negative results and ensured the accuracy of the duplex-rtRAA system. We first developed and evaluated a universal singleplex-rtRAA assay for RSV. The sensitivity of this assay for RSV was determined as 4.4 copies per reaction, and the specificity was 100%. Next, a duplex-rtRAA assay with an internal control was established. The sensitivity of the duplex-rtRAA assay approached 5.0 copies per reaction, and no cross-reaction with other common respiratory viruses was observed. The two detection methods (singleplex-rtRAA and duplex-rtRAA) developed in this study were used to test 278 clinical specimens, and the results showed absolute consistency with RSV RT-qPCR analysis, demonstrating 100% diagnostic sensitivity and specificity. These data indicate that the duplex-rtRAA has great potential for the rapid detection of RSV with a high sensitivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.