Evidence map›Paper›PMID 31053882›Full record

ArticleEuropean journal of nutrition2020

Plasma concentrations and ACE-inhibitory effects of tryptophan-containing peptides from whey protein hydrolysate in healthy volunteers.

Melanie Martin, Diana Hagemann, Thinh Trung Nguyen, Lisa Schwarz, Sherif Khedr, Mats Leif Moskopp, Thomas Henle, Andreas Deussen

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In one paragraph

Article in European journal of nutrition, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.

  1. Pooled it
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  3. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Melanie MartinInstitute of Physiology, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, 01307, Dresden, Germany. melanie.martin@tu-dresden.de.ORCID http://orcid.org/0000-0003-2524-3124
Diana HagemannChair of Food Chemistry, Technische Universität Dresden, 01062, Dresden, Germany.
Thinh Trung NguyenChair of Food Chemistry, Technische Universität Dresden, 01062, Dresden, Germany.
Lisa SchwarzChair of Food Chemistry, Technische Universität Dresden, 01062, Dresden, Germany.
Sherif KhedrInstitute of Physiology, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, 01307, Dresden, Germany.
Mats Leif MoskoppInstitute of Physiology, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, 01307, Dresden, Germany.
Thomas HenleChair of Food Chemistry, Technische Universität Dresden, 01062, Dresden, Germany.
Andreas DeussenInstitute of Physiology, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, 01307, Dresden, Germany.
Technische Universität Dresden · DEUniversity Hospital Carl Gustav Carus · DE

Funding

Bundesministerium für Bildung und Forschung 03V0538
6 · The paper itself

Abstract

purposeThe tryptophan-containing dipeptides isoleucine-tryptophan (IW) and tryptophan-leucine (WL) are angiotensin-converting enzyme (ACE)-inhibitors in vitro. These peptides are released by enzymatic hydrolysis of bovine whey protein. To exhibit ACE inhibition in vivo, peptides need to be absorbed into the circulatory system. This study aimed to determine the in vivo ACE-inhibitory potency of a whey protein hydrolysate (MPH), containing IW and WL, and to quantify plasma concentrations of these peptides after oral administration of MPH in healthy volunteers. Additionally, changes in blood pressure were investigated.

resultsAfter intake of 5 and 50 g MPH, plasma ACE activity was reduced to 86.4 ± 5.9 and 75.1 ± 6.9% of baseline activity, respectively. Although a clear ACE inhibition was measured, no effect on blood pressure was seen. Basal plasma concentrations of the tryptophan-containing dipeptides were 2.8 ± 0.7 nM for IW and 10.1 ± 1.8 nM for WL. After intake of 5-50 g MPH, peptide concentrations were dose dependently elevated to values between 12.5 ± 8.4 and 99.1 ± 58.7 nM for IW and 15.0 ± 4.3-34.9 ± 19.4 nM for WL. Administration of intact whey protein showed a minor ACE inhibition, probably caused by release of inhibitory peptides during gastrointestinal digestion. The increase of WL in plasma after intake of intact protein was similar to that determined after intake of MPH. In contrast, resulting IW concentrations were much lower after intake of intact whey protein when compared to MPH administration.

conclusionAfter intake of MPH, plasma ACE activity decreased in parallel to the increase of IW and WL plasma concentrations. However, the resulting peptide concentrations cannot fully explain the reduction of ACE activity in plasma with a direct enzyme inhibition. Therefore, this study points to a gap in the understanding of the inhibitory action of these peptides in vivo. Thus, to further develop innovative food additives with ACE activity diminishing capabilities, it appears mandatory to better characterize the mode of action of these peptides.

Indexed as

AdultAngiotensin-Converting Enzyme InhibitorsBlood PressureFemaleHealthy VolunteersHumansMaleMiddle AgedPilot ProjectsProtein HydrolysatesReference ValuesSingle-Blind MethodTryptophanWhey ProteinsAngiotensin-Converting Enzyme InhibitorsProtein HydrolysatesTryptophanWhey ProteinsACE-inhibitory peptidesAngiotensin converting enzymeAntihypertensive peptidesArterial hypertensionWhey protein

Identifiers

PMID31053882
OpenAlexW2943156037

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.