ArticleMolecular systems biology2019
Absolute quantification of translational regulation and burden using combined sequencing approaches.
Article in Molecular systems biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 67 citations in OpenAlex.
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- Technologies for whole-cell modeling: Genome-wide reconstruction of a cell in silico.Development, growth & differentiation · 2023Review
- Comprehensive quantitative modeling of translation efficiency in a genome-reduced bacterium.Molecular systems biology · 2023Article
- Design and Analysis of Massively Parallel Reporter Assays Using FORECAST.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Absolute protein quantification using fluorescence measurements with FPCountR.Nature communications · 2022Article
- Massively parallel characterization of engineered transcript isoforms using direct RNA sequencing.Nature communications · 2022Article
- The context matrix: Navigating biological complexity for advanced biodesign.Frontiers in bioengineering and biotechnology · 2022Article
- RBS and Promoter Strengths Determine the Cell-Growth-Dependent Protein Mass Fractions and Their Optimal Synthesis Rates.ACS synthetic biology · 2021Article
- Context-Dependent Stability and Robustness of Genetic Toggle Switches with Leaky Promoters.Life (Basel, Switzerland) · 2021Article
- Quantitative Control for Stoichiometric Protein Synthesis.Annual review of microbiology · 2021Article
- Article
- Design patterns for engineering genetic stability.Current opinion in biomedical engineering · 2021Article
- Towards the characterization of the hidden world of small proteins in Staphylococcus aureus, a proteogenomics approach.PLoS genetics · 2021Article
- Stability and Robustness of Unbalanced Genetic Toggle Switches in the Presence of Scarce Resources.Life (Basel, Switzerland) · 2021Article
- Single-cell measurement of plasmid copy number and promoter activity.Nature communications · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Translation of mRNAs into proteins is a key cellular process. Ribosome binding sites and stop codons provide signals to initiate and terminate translation, while stable secondary mRNA structures can induce translational recoding events. Fluorescent proteins are commonly used to characterize such elements but require the modification of a part's natural context and allow only a few parameters to be monitored concurrently. Here, we combine Ribo-seq with quantitative RNA-seq to measure at nucleotide resolution and in absolute units the performance of elements controlling transcriptional and translational processes during protein synthesis. We simultaneously measure 779 translation initiation rates and 750 translation termination efficiencies across the
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.