ArticleOptics express2019
Measurement of the relative non-degenerate two-photon absorption cross-section for fluorescence microscopy.
Article in Optics express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Non-degenerate two-photon imaging of deep rodent cortex using indocyanine green in the water absorption window.Biomedical optics express · 2024Article
- Non-Degenerate Two-Photon Imaging of Deep Rodent Cortex using Indocyanine Green in the water absorption window.bioRxiv : the preprint server for biology · 2024Article
- Diamond Raman laser and Yb fiber amplifier forBiomedical optics express · 2022Article
- Neurophotonic tools for microscopic measurements and manipulation: status report.Neurophotonics · 2022Article
- Comparing the fundamental imaging depth limit of two-photon, three-photon, and non-degenerate two-photon microscopy.Optics letters · 2020Article
- Efficient non-degenerate two-photon excitation for fluorescence microscopy.Optics express · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
In non-degenerate two-photon microscopy (ND-TPM), the required energy for fluorescence excitation occurs via absorption of two photons of different energies derived from two synchronized pulsed laser beams. ND-TPM is a promising imaging technology offering flexibility in the choice of the photon energy for each beam. However, a formalism to quantify the efficiency of two-photon absorption (TPA) under non-degenerate excitation, relative to the resonant degenerate excitation, is missing. Here, we derive this formalism and experimentally validate our prediction for a common fluorophore, fluorescein. An accurate quantification of non-degenerate TPA is important to optimize the choice of photon energies for each fluorophore.
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Registered trials
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