ArticlePolymers2019
Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean (
Article in Polymers, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 29 citations in OpenAlex.
- Purification of Food Allergens from Their Natural Sources: Chromatographic Methods.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Aptamer-functionalized stir bar sorptive extraction for selective isolation, identification, and determination of concanavalin A in food by MALDI-TOF-MS.Mikrochimica acta · 2023Article
- Partial Characterization of Lectins Purified from the Surco and Vara (Furrow and Rod) Varieties of BlackMolecules (Basel, Switzerland) · 2022Article
- Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).Molecular biotechnology · 2022Article
- Article
- Efficacy of Live Attenuated Vaccine and Commercially Available Lectin Against Avian PathogenicVeterinary sciences · 2020Article
- Study on the Preparation and Conjugation Mechanism of the Phosvitin-Gallic Acid Complex with an Antioxidant and Emulsifying Capability.Polymers · 2019Article
- Ultrasonic Thermal-Assisted Extraction of Phosvitin from Egg Yolk and Evaluation of Its Properties.Polymers · 2019Article
- Reutilization of Food Waste: One-Step Extration, Purification and Characterization of Ovalbumin from Salted Egg White by Aqueous Two-Phase Flotation.Foods (Basel, Switzerland) · 2019Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
A two-step method based on an aqueous two-phase system and Sephadex G-75 was used to separate and purify lectin from the seeds of the Zihua snap bean. The preliminary properties and bioactivity of the Zihua snap bean lectin were characterized by different instrumental methods, such as sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), liquid chromatography-nano electrospray ionization mass spectrometry (Nano LC-ESI-MS/MS), and Fourier transform infrared spectroscopy (FTIR). The hemagglutinating activity of the Zihua snap bean lectin could not be inhibited by glucose, N-acetyl-D-glucosamine, D-galactose, N-acetyl-D-galactosamine, fructose, sucrose, D-maltose, D-trehalose, and lactose. It was found that the hemagglutinating activity of the lectin showed strong dependence on Mn
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