ArticleACS synthetic biology2019
Controlling Secretion in Artificial Cells with a Membrane AND Gate.
Article in ACS synthetic biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Synthetic cells by the numbers.iScience · 2025Review
- pH-responsive synthetic cells for controlled protein synthesis and release.bioRxiv : the preprint server for biology · 2025Article
- Strategies and applications of synthetic cell communication.Nature chemical biology · 2025Review
- Light-Triggered Protease-Mediated Release of Actin-Bound Cargo from Synthetic Cells.Advanced biology · 2025Article
- Structural insights into lab-coevolved RNA-RBP pairs and applications of synthetic riboswitches in cell-free system.Nucleic acids research · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Phenogenetics of cortical granule dynamics during zebrafish oocyte-to-embryo transition.Frontiers in cell and developmental biology · 2025Review
- Genetically programmed synthetic cells for thermo-responsive protein synthesis and cargo release.Nature chemical biology · 2024Article
- Light-triggered protease-mediated release of actin-bound cargo from synthetic cells.bioRxiv : the preprint server for biology · 2024Article
- Manipulation of encapsulated artificial phospholipid membranes using sub-micellar lysolipid concentrations.Communications chemistry · 2024Article
- Hydrophobic mismatch drives self-organization of designer proteins into synthetic membranes.Nature communications · 2024Article
- Rapid Generation of Therapeutic Nanoparticles Using Cell-Free Expression Systems.Small methods · 2023Article
- Biomimetic behaviors in hydrogel artificial cells through embedded organelles.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many.ACS synthetic biology · 2023Review
- Engineering transmembrane signal transduction in synthetic membranes using two-component systems.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Light-Activated Assembly of Connexon Nanopores in Synthetic Cells.Journal of the American Chemical Society · 2023Article
- Robust and tunable performance of a cell-free biosensor encapsulated in lipid vesicles.Science advances · 2023Article
- DNA-Mediated Protein Shuttling between Coacervate-Based Artificial Cells.Angewandte Chemie (International ed. in English) · 2022Article
- Vesicle encapsulation stabilizes intermolecular association and structure formation of functional RNA and DNA.Current biology : CB · 2022Article
- Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The assembly of channel proteins into vesicle membranes is a useful strategy to control activities of vesicle-based systems. Here, we developed a membrane AND gate that responds to both a fatty acid and a pore-forming channel protein to induce the release of encapsulated cargo. We explored how membrane composition affects the functional assembly of α-hemolysin into phospholipid vesicles as a function of oleic acid content and α-hemolysin concentration. We then showed that we could induce α-hemolysin assembly when we added oleic acid micelles to a specific composition of phospholipid vesicles. Finally, we demonstrated that our membrane AND gate could be coupled to a gene expression system. Our study provides a new method to control the temporal dynamics of vesicle permeability by controlling when the functional assembly of a channel protein into synthetic vesicles occurs. Furthermore, a membrane AND gate that utilizes membrane-associating biomolecules introduces a new way to implement Boolean logic that should complement genetic logic circuits and ultimately enhance the capabilities of artificial cellular systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.