ArticlePloS one2019
Reliability of blood lactate as a measure of exercise intensity in different strains of mice during forced treadmill running.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Preclinical techniques to investigate exercise training in vascular pathophysiology.American journal of physiology. Heart and circulatory physiology · 2021Pooled it
- Exercise-induced protein lactylation: From molecular mechanisms to precision health applications.iScience · 2026Review
- Acute exercise-induced lipolysis and increase in levels of fatty acid esters of hydroxy fatty acids in adipose tissue are impaired in obese and aged mice.Experimental physiology · 2026Article
- Glycyrrhizic Acid Relieves Fatigue Induced by Acute and Chronic Exercise in Mice via Inhibition of the NF-κB/NLRP3 Signaling Pathway.Brain and behavior · 2026Article
- Energy Substrate Utilization and Body Composition Following Exercise Preconditioning During Alternate-Day Fasting and Concurrent Exercise in Mice.Metabolites · 2026Article
- Article
- Neuronal MCT2 promotes angiogenesis via lactate in the developing mouse neocortex.Cell death and differentiation · 2026Article
- Establishing VO2peak-based exercise intensity classification and physiological validation in ICR mice.Physical activity and nutrition · 2025Article
- Role of Histone Lactylation in Neurological Disorders.International journal of molecular sciences · 2025Review
- Effects of aerobic and anaerobic exercise on glucose, lipid, and inflammation-related gene expression in the brain tissue of streptozotocin-induced diabetic rat model.Diabetology & metabolic syndrome · 2025Article
- Blood lactate as a marker of exercise adaptation and fatigue in male mice with chronic heart failure.Physiological reports · 2025Article
- Various endurance training intensities improve GFR and Up-regulate AQP2/GSK3β in lithium-induced nephropathic rats.BMC nephrology · 2025Article
- Moderate-intensity training can ameliorate the process of cardiac apoptosis induced by lithium drug consumption in male Wistar rats.Toxicology reports · 2024Article
- Exercise improves cognitive dysfunction and neuroinflammation in mice through Histone H3 lactylation in microglia.Immunity & ageing : I & A · 2023Article
- Endurance training and MitoQ supplementation improve spatial memory, VEGF expression, and neurogenic factors in hippocampal tissue of rats.Journal of clinical and translational research · 2023Article
- Genetically encoded tools for measuring and manipulating metabolism.Nature chemical biology · 2022Review
- A Formalized Method to Acclimate Dogs to Voluntary Treadmill Locomotion at Various Speeds and Inclines.Animals : an open access journal from MDPI · 2022Article
- Validity of the peak velocity to detect physical training improvements in athymic mice.Frontiers in physiology · 2022Article
- Barriers in translating preclinical rodent exercise metabolism findings to human health.Journal of applied physiology (Bethesda, Md. : 1985) · 2021Article
- Systematic Review and Meta-Analysis of Endurance Exercise Training Protocols for Mice.Frontiers in physiology · 2021Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exercise has long been known to be beneficial to human health. Studies aimed at understanding the effects of exercise specifically focus on predetermined exercise intensities defined by measuring the aerobic capacity of each individual. Many disease models involving animal training often establish aerobic capacity by using the maximal lactate steady state (MLSS), a widely used method in humans that has frequently been used in rodent studies. The MLSS is defined as the highest exercise intensity at which blood lactate concentration remains constant and is roughly equivalent to 70-80% of maximal aerobic capacity. Due to our up-coming experiments investigating the effect of different exercise intensities in specific strains of tumor-bearing mice, the aim of the present study was to determine the MLSS in athymic nude (NCr nu/nu and NMRI), CDF1, and C3H mice by treadmill running at increasing speeds. However, despite thorough exercise acclimation and the use of different exercise protocols and aversive stimuli, less than half of the experiments across strains pointed towards an established MLSS. Moreover, gently prodding the mice during low to moderate intensity running caused a 30-121% (p<0.05) increase in blood lactate concentration compared to running without stimulation, further questioning the use of lactate as a measure of exercise intensity. Overall, MLSS is difficult to determine and large variations of blood lactate levels were observed depending on the exercise protocol, mice handling strategy and strain. This should be considered when planning experiments in mice using forced exercise protocols.
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