ArticleAnalytical chemistry2019
Label-Free, Visual Detection of Small Molecules Using Highly Target-Responsive Multimodule Split Aptamer Constructs.
Article in Analytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 75 citations in OpenAlex.
- A Label-Free Colorimetric Aptasensor for Flavokavain B Detection.Sensors (Basel, Switzerland) · 2025Article
- Nuclease-assisted selection of slow-off rate aptamers.Science advances · 2024Article
- Recent reports on the sensing strategy and the On-site detection of illegal drugs.RSC advances · 2024Review
- Aptasensors Based on Non-Enzymatic Peroxidase Mimics: Current Progress and Challenges.Biosensors · 2023Review
- Molecular Docking Insight into the Label-Free Fluorescence Aptasensor for Ochratoxin A Detection.Molecules (Basel, Switzerland) · 2023Article
- Improving aptamer performance: key factors and strategies.Mikrochimica acta · 2023Review
- On the Rational Design of Cooperative Receptors.Annual review of biophysics · 2023Review
- Interpol Review of Drug Analysis 2019-2022.Forensic science international. Synergy · 2023Review
- A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer.Sensors and actuators reports · 2022Article
- Colorimetric and fluorescent detection of synthetic cathinones in oral fluid withRSC advances · 2022Article
- Metabolomics for personalized medicine: the input of analytical chemistry from biomarker discovery to point-of-care tests.Analytical and bioanalytical chemistry · 2022Review
- DNA Nanodevice-Based Drug Delivery Systems.Biomolecules · 2021Review
- Advances and Challenges in Small-Molecule DNA Aptamer Isolation, Characterization, and Sensor Development.Angewandte Chemie (International ed. in English) · 2021Review
- Using Simple-Structured Split Aptamer for Gold Nanoparticle-based Colorimetric Detection of Estradiol.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- Nucleic-Acid Driven Cooperative Bioassays Using Probe Proximity or Split-Probe Techniques.Analytical chemistry · 2021Review
- Label-free profiling of DNA aptamer-small molecule binding using T5 exonuclease.Nucleic acids research · 2020Article
- Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors.Nucleic acids research · 2020Review
- Toward a Rational Approach to Design Split G-Quadruplex Probes.ACS chemical biology · 2019Article
- Innovative engineering and sensing strategies for aptamer-based small-molecule detection.Trends in analytical chemistry : TRAC · 2019Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
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Abstract
Colorimetric aptamer-based sensors offer a simple means of on-site or point-of-care analyte detection. However, these sensors are largely incapable of achieving naked-eye detection, because of the poor performance of the target-recognition and signal-reporting elements employed. To address this problem, we report a generalizable strategy for engineering novel multimodule split DNA constructs termed "CBSAzymes" that utilize a cooperative binding split aptamer (CBSA) as a highly target-responsive bioreceptor and a new, highly active split DNAzyme as an efficient signal reporter. CBSAzymes consist of two fragments that remain separate in the absence of target, but effectively assemble in the presence of the target to form a complex that catalyzes the oxidation of 2,2'-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid, developing a dark green color within 5 min. Such assay enables rapid, sensitive, and visual detection of small molecules, which has not been achieved with any previously reported split-aptamer-DNAzyme conjugates. In an initial demonstration, we generate a cocaine-binding CBSAzyme that enables naked-eye detection of cocaine at concentrations as low as 10 μM. Notably, CBSAzyme engineering is straightforward and generalizable. We demonstrate this by developing a methylenedioxypyrovalerone (MDPV)-binding CBSAzyme for visual detection of MDPV and 10 other synthetic cathinones at low micromolar concentrations, even in biological samples. Given that CBSAzyme-based assays are simple, label-free, rapid, robust, and instrument-free, we believe that such assays should be readily applicable for on-site visual detection of various important small molecules such as illicit drugs, medical biomarkers, and toxins in various sample matrices.
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Registered trials
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