ReviewProteomics2019
Identification and Quantification of Proteoforms by Mass Spectrometry.
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In one paragraph
Review in Proteomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 117 papers.
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117citing papers in PubMed
–field-weighted citation impact
What it found
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Who cites it
117 citing papers in PubMed.
- Multi-omics analyses based identification of therapeutic targets for rapid kidney function decline and chronic kidney disease, a mendelian randomization study.International urology and nephrology · 2026Article
- Canine Tear Proteomics: A New Frontier in Veterinary Ophthalmology.Veterinary ophthalmology · 2026Review
- Native Multidimensional Protein Complex Identification and Topology Characterization (Native MudPIT).Analytical chemistry · 2026Article
- Top-Down Proteomics of Zebrafish Brain Regions Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry.Journal of proteome research · 2026Article
- Top-Down Mass Spectrometry and Its Current Applications in Biomarker Discovery in Aging and Age-Related Diseases.International journal of molecular sciences · 2026Review
- Article
- Proteoform medicine: characterizing and targeting protein forms in human disease.Nature reviews. Genetics · 2026Review
- Mass spectrometry-based top-down proteomics for proteoform profiling of protein coronas.Nature protocols · 2026Review
- Structural mass spectrometry techniques for characterisation of plant and algal proteins.The Plant journal : for cell and molecular biology · 2026Review
- ProteoformDB: A Built-In Application to Generate Proteoform Database.Journal of proteome research · 2026Article
- Review
- Evaluation of Protein Ion Relative Ratio Quantification in Top-down Electrospray Ionization-Mass Spectrometry Using Site-Specific Acetylated Recombinant Histone H3 Proteoforms.Journal of the American Society for Mass Spectrometry · 2025Article
- Identifying lncRNA-Protein Interactions in Hematopoietic Progenitor Cells by Hybridization Capture and Mass Spectrometry.Journal of proteome research · 2025Article
- Developments in Ion Exchange Chromatography-Mass Spectrometry for the Characterization of Intact Proteins and Proteoforms.Journal of separation science · 2025Review
- HDL proteome: from its characterization to quantitative measurements in particle subspecies and functional associations.Expert review of proteomics · 2025Review
- TopLib: Building and Searching Top-Down Mass Spectral Libraries for Proteoform Identification.Analytical chemistry · 2025Article
- PEPPI-MS: gel-based sample pre-fractionation for deep top-down and middle-down proteomics.Nature protocols · 2025Review
- Capillary Electrophoresis-Mass Spectrometry for Top-Down Proteomics.Annual review of analytical chemistry (Palo Alto, Calif.) · 2025Review
- Integrating the milk microbiome signatures in mastitis: milk-omics and functional implications.World journal of microbiology & biotechnology · 2025Review
- Capillary Isoelectric Focusing of Proteins and Peptides Using an In-Line cIEF-ESI Interface with Improved MS Characteristics.Analytical chemistry · 2025Article
57 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
19 authors.
Leah V SchafferDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Robert J MillikinDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Rachel M MillerDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Lissa C AndersonIon Cyclotron Resonance Program, National High Magnetic Field Laboratory, Tallahassee, FL, 32310, USA.
Ryan T FellersProteomics Center of Excellence, Northwestern University, Evanston, IL, 60208, USA.
Ying GeDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Neil L KelleherProteomics Center of Excellence, Northwestern University, Evanston, IL, 60208, USA.
Richard D LeDucProteomics Center of Excellence, Northwestern University, Evanston, IL, 60208, USA.
Xiaowen LiuDepartment of BioHealth Informatics, Indiana University-Purdue University, Indianapolis, IN, 46202, USA.
Samuel H PayneDepartment of Biology, Brigham Young University, Provo, UT, 84602.
Liangliang SunDepartment of Chemistry, Michigan State University, East Lansing, MI, 48824, USA.
Paul M ThomasProteomics Center of Excellence, Northwestern University, Evanston, IL, 60208, USA.
Trisha TucholskiDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Zhe WangDepartment of Chemistry and Biochemistry, University of Oklahoma, Norman, OK, 73019, USA.
Si WuDepartment of Chemistry and Biochemistry, University of Oklahoma, Norman, OK, 73019, USA.
Zhijie WuDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Dahang YuDepartment of Chemistry and Biochemistry, University of Oklahoma, Norman, OK, 73019, USA.
Michael R ShortreedDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Lloyd M SmithDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Funding
BIOTECHNOLOGY TRAINING PROGRAMT32GM008349 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI FOX, BRIAN G · 1989 to 2019
$22.5MInstitutional Training in the Genomic SciencesT32HG002760 · NHGRI · UNIVERSITY OF WISCONSIN-MADISON · PI Qiongshi Lu · 2003 to 2026
$17.7MTR&D 7: Cell Specific ProteomicsP41GM108569 · NIGMS · NORTHWESTERN UNIVERSITY · PI KELLEHER, NEIL L · 2015 to 2024
$13.6MChemistry-Biology Interface Training ProgramT32GM008505 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI BLACKWELL, HELEN E. · 1993 to 2023
$9.8MRevealing Proteoforms: The Primary Effectors of Biological FunctionR35GM126914 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Lloyd M Smith · 2018 to 2026
$6.2MDeciphering Myofilament Modifications in Ischemic CardiomyopathyR01HL109810 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING · 2013 to 2024
$3.5MTop Down Proteomics Of Myofilaments In Heart FailureR01HL096971 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING · 2011 to 2021
$3.3MComputational tools for top down mass spectrometry based proteoform identification and proteogenomicsR01GM118470 · NIGMS · TULANE UNIVERSITY OF LOUISIANA · PI Xiaowen Liu · 2016 to 2026
$2.7MEnabling Top-Down Proteomics through Materials Chemistry and NanotechnologyR01GM117058 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING, JIN, SONG · 2015 to 2023
$2.7MUltra High Resolution Mass Spectrometer for Biomedical ResearchS10OD018475 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING · 2015 to 2015
$2.0MQuantitative proteomics of Zebrafish embryos and blastomeresR01GM125991 · NIGMS · MICHIGAN STATE UNIVERSITY · PI SUN, LIANGLIANG · 2018 to 2022
$1.7MIntact Proteoform Identification and QuantificationR01GM114292 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI GOULD, MICHAEL N, SMITH, LLOYD M · 2015 to 2018
$1.6MNHGRI NIH HHS 5T32HG002760NHGRI NIH HHS T32 HG002760NHLBI NIH HHS R01 HL096971NHLBI NIH HHS R01 HL109810NIGMS NIH HHS P41 GM108569NIGMS NIH HHS P41GM108569NIGMS NIH HHS R01 GM114292NIGMS NIH HHS R01GM114292NIGMS NIH HHS R01 GM117058NIGMS NIH HHS R01 GM118470NIGMS NIH HHS R01 GM125085NIGMS NIH HHS R01 GM125991NIGMS NIH HHS R35 GM126914NIGMS NIH HHS R35GM126914NIGMS NIH HHS T32 GM008349NIGMS NIH HHS T32GM008349NIGMS NIH HHS T32 GM008505NIGMS NIH HHS T32GM008505NIH HHS R01GM117058NIH HHS R01GM118470NIH HHS R01GM125085NIH HHS R01GM125991NIH HHS R01HL096971NIH HHS R01HL109810NIH HHS S10 OD018475NIH HHS S10OD018475
Abstract
A proteoform is a defined form of a protein derived from a given gene with a specific amino acid sequence and localized post-translational modifications. In top-down proteomic analyses, proteoforms are identified and quantified through mass spectrometric analysis of intact proteins. Recent technological developments have enabled comprehensive proteoform analyses in complex samples, and an increasing number of laboratories are adopting top-down proteomic workflows. In this review, some recent advances are outlined and current challenges and future directions for the field are discussed.
Indexed as
Mass SpectrometryProtein Processing, Post-TranslationalAmino AcidsAnimalsComputational BiologyElectrophoresis, CapillaryHumansProgramming LanguagesProteomeProteomicsReproducibility of ResultsSoftwareAmino AcidsProteomebioinformaticsmass spectrometryproteoformproteoform familytop-down proteomics
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.