ArticleMikrochimica acta2019
A fluorometric clenbuterol immunoassay using sulfur and nitrogen doped carbon quantum dots.
Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 31 citations in OpenAlex.
- Carbon quantum dots with blue/near infrared emissions for ratiometric fluorescent lornoxicam sensing and bio-imaging.Mikrochimica acta · 2022Article
- Ratiometric Fluorescent Biosensor Based on Forster Resonance Energy Transfer between Carbon Dots and Acridine Orange for miRNA Analysis.ACS omega · 2021Article
- Facile synthesis of carbon nitride quantum dots as a highly selective and sensitive fluorescent sensor for the tetracycline detection.RSC advances · 2021Article
- A Multi-Catalytic Sensing for Hydrogen Peroxide, Glucose, and Organophosphorus Pesticides Based on Carbon Dots.Frontiers in chemistry · 2021Article
- Improved food additive analysis by ever-increasing nanotechnology.Journal of food and drug analysis · 2020Review
- Synthesis, Properties, and Biological Applications of Metallic Alloy Nanoparticles.International journal of molecular sciences · 2020Review
- Biosensors Based on Advanced Sulfur-Containing Nanomaterials.Sensors (Basel, Switzerland) · 2020Review
- A Perspective on Application of Carbon Quantum Dots in Luminescence Immunoassays.Frontiers in chemistry · 2020Article
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3 authors at 2 institutions in 1 country.
Funding
Abstract
A fluorometric clenbuterol immunoassay is described that uses S- and N-co-doped carbon quantum dots as the fluorescent probe. Strongly fluorescent S/N-doped carbon quantum dots (S/N-CDs) were synthesized by hydrothermal method using fructose as the carbon precursor and L-cysteine as S/N sources. The S/N-CDs were characterized by transmission electron microscopy, energy dispersive spectroscopy and Fourier transform infrared spectroscopy (FTIR). Under 350 nm photoexcitation, they display strong purple fluorescence with an emission peak at 405 nm. In pH 4.0 solution, the amino groups (confirmed by FTIR) on the carbon quantum dots were coupled to clenbuterol antibody (Ab) by amine-amine coupling reaction to quench the fluorescence. If clenbuterol (Clen) is added, it binds to the Ab to generate a stable Ab-Clen immunocomplex and free S/N-CD. This causes the fluorescence of nanoprobe to be restored. The fluorescence of the system increases linearly in the 0.07-1.7 ng·mL
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