ArticleGigaScience2019
Single-cell transcriptomic landscape of nucleated cells in umbilical cord blood.
Article in GigaScience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Integrating single-cell RNA and T cell/B cell receptor sequencing with mass cytometry reveals dynamic trajectories of human peripheral immune cells from birth to old age.Nature immunology · 2025Observational
- Immunophenotypic and Functional Interindividual Variability in Banked Cord Blood Cells: Insights for Advanced Therapies.International journal of molecular sciences · 2025Article
- Immune composition of the mononuclear cell fraction of human umbilical cord blood.Frontiers in immunology · 2025Article
- CRISPR Dependency Screens in Primary Hematopoietic Stem Cells Identify KDM3B as a Genotype-specific Vulnerability in IDH2- and TET2-mutant Cells.Cancer discovery · 2024Article
- Single-Cell RNA Sequencing in Organ and Cell Transplantation.Biosensors · 2024Review
- Macrophage and neutrophil heterogeneity at single-cell spatial resolution in human inflammatory bowel disease.Nature communications · 2023Article
- Article
- Placental cell type deconvolution reveals that cell proportions drive preeclampsia gene expression differences.Communications biology · 2023Article
- Origin, prospective identification, and function of circulating endothelial colony-forming cells in mice and humans.JCI insight · 2023Article
- Single-cell immunophenotyping of the fetal immune response to maternal SARS-CoV-2 infection in late gestation.Pediatric research · 2022Article
- Single-cell entropy network detects the activity of immune cells based on ribosomal protein genes.Computational and structural biotechnology journal · 2022Article
- The Distinct Immune Nature of the Fetal Inflammatory Response Syndrome Type I and Type II.ImmunoHorizons · 2021Article
- Temporal omics analysis in Syrian hamsters unravel cellular effector responses to moderate COVID-19.Nature communications · 2021Article
- Utilizing single-cell RNA sequencing for analyzing the characteristics of PBMC in patients with Kawasaki disease.BMC pediatrics · 2021Article
- Single-cell immunophenotyping of the fetal immune response to maternal SARS-CoV-2 infection in late gestation.Research square · 2021Article
- Single-cell resolution landscape of equine peripheral blood mononuclear cells reveals diverse cell types including T-betBMC biology · 2021Article
- Dissecting the Landscape of Activated CMV-Stimulated CD4+ T Cells in Humans by Linking Single-Cell RNA-Seq With T-Cell Receptor Sequencing.Frontiers in immunology · 2021Article
- The Presence of Circulating Nucleated Red Blood Cells Is Associated With Disease Severity in Patients of Hemorrhagic Fever With Renal Syndrome.Frontiers in medicine · 2021Article
- Article
- SEQdata-BEACON: a comprehensive database of sequencing performance and statistical tools for performance evaluation and yield simulation in BGISEQ-500.BioData mining · 2019Article
Corrections and comments
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Authors and funding
23 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFor both pediatric and adult patients, umbilical cord blood (UCB) transplant is a therapeutic option for a variety of hematologic diseases, such as blood cancers, myeloproliferative disorders, genetic diseases, and metabolic disorders. However, the level of cellular heterogeneity and diversity of nucleated cells in UCB has not yet been assessed in an unbiased and systemic fashion. In the present study, nucleated cells from UCB were subjected to single-cell RNA sequencing to simultaneously profile the gene expression signatures of thousands of cells, generating a rich resource for further functional studies. Here, we report the transcriptomes of 17,637 UCB cells, covering 12 major cell types, many of which can be further divided into distinct subpopulations.
resultsPseudotemporal ordering of nucleated red blood cells identifies wave-like activation and suppression of transcription regulators, leading to a polarized cellular state, which may reflect nucleated red blood cell maturation. Progenitor cells in UCB also comprise 2 subpopulations with activation of divergent transcription programs, leading to specific cell fate commitment. Detailed profiling of cytotoxic cell populations unveiled granzymes B and K signatures in natural killer and natural killer T-cell types in UCB.
conclusionsTaken together, our data form a comprehensive single-cell transcriptomic landscape that reveals previously unrecognized cell types, pathways, and mechanisms of gene expression regulation. These data may contribute to the efficacy and outcome of UCB transplant, broadening the scope of research and clinical innovations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.