Evidence map›Paper›PMID 31049303›Full record

ArticleAfrican journal of laboratory medicine2019

Implementation and evaluation of the Presto combined qualitative real-time assay for

Vicky Cuylaerts, Irith De Baetselier, Claude M Muvunyi, Lambert Mwambarange, Hilde Smet, John Rusine, Viateur Musengamana, Janneke van de Wijgert, Tania Crucitti

Open access · diamondAbstract read
In one paragraph

Article in African journal of laboratory medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 3 countries.

Vicky CuylaertsInstitute of Tropical Medicine, Department of Clinical Sciences, STI Reference Laboratory, Antwerp, Belgium.ORCID https://orcid.org/0000-0003-0183-0033
Irith De BaetselierInstitute of Tropical Medicine, Department of Clinical Sciences, STI Reference Laboratory, Antwerp, Belgium.ORCID https://orcid.org/0000-0002-1804-252X
Claude M MuvunyiCollege of Medicine and Health Sciences, University of Rwanda, Kigali, Rwanda.ORCID https://orcid.org/0000-0003-4180-442X
Lambert MwambarangeLegacy Clinics and Diagnostics, Kigali, Rwanda.ORCID https://orcid.org/0000-0002-1393-4545
Hilde SmetInstitute of Tropical Medicine, Department of Clinical Sciences, STI Reference Laboratory, Antwerp, Belgium.ORCID https://orcid.org/0000-0002-9374-2710
John RusineNational Reference Laboratory, Ministry of Health, Kigali, Rwanda.ORCID https://orcid.org/0000-0001-8519-0840
Viateur MusengamanaRinda Ubuzima, Kigali, Rwanda.ORCID https://orcid.org/0000-0002-6512-5467
Janneke van de WijgertDepartment of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom.ORCID https://orcid.org/0000-0003-2728-4560
Tania CrucittiInstitute of Tropical Medicine, Department of Clinical Sciences, STI Reference Laboratory, Antwerp, Belgium.ORCID https://orcid.org/0000-0002-2235-6038
Instituut voor Tropische Geneeskunde · BEMinistry of Health · RWRwanda Biomedical Center · RWUniversity of Liverpool · GBUniversity of Rwanda · RW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe Presto combined qualitative real-time assay for

objectivesThe objective of the study was to implement and evaluate the Presto CT/NG PCR assay at the National Reference Laboratory (NRL) in Kigali, Rwanda, where no real-time PCR assays for the detection of

methodsThe Presto CT/NG PCR assay was first evaluated at the Institute of Tropical Medicine (ITM) in Antwerp, Belgium. Next, NRL laboratory technicians were trained to use the assay on their ABI PRISM 7500 real-time PCR instrument and their competencies were assessed prior to trial initiation. During the trial, endocervical swabs were tested at the NRL, with bi-monthly external quality control testing monitored by the ITM. The final NRL results were evaluated against extended gold standard testing at the ITM, consisting of the Abbott

resultsOf the 192 samples analysed using the Presto assay at the NRL, 16 samples tested positive for

conclusionKEYWORDS: qPCR;

Identifiers

PMID31049303
PMCPMC6489157
OpenAlexW2939940150

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.