Evidence map›Paper›PMID 31049301›Full record

ArticleBiotechnology reports (Amsterdam, Netherlands)2019

Design of a chromogenic substrate for elastase based on split GFP system-Proof of concept for colour switch sensors.

Ana V Ferreira, Egipto Antunes, Artur Ribeiro, Teresa Matamá, Nuno G Azoia, Joana Cunha, Artur Cavaco-Paulo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology reports (Amsterdam, Netherlands), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Ana V FerreiraCentre of Biological Engineering (CEB), University of Minho, Campus of Gualtar, 4710-057, Braga, Portugal.
Egipto AntunesCentre of Biological Engineering (CEB), University of Minho, Campus of Gualtar, 4710-057, Braga, Portugal.
Artur RibeiroCentre of Biological Engineering (CEB), University of Minho, Campus of Gualtar, 4710-057, Braga, Portugal.
Teresa MatamáCentre of Biological Engineering (CEB), University of Minho, Campus of Gualtar, 4710-057, Braga, Portugal.
Nuno G AzoiaCentre of Biological Engineering (CEB), University of Minho, Campus of Gualtar, 4710-057, Braga, Portugal.
Joana CunhaCentre of Biological Engineering (CEB), University of Minho, Campus of Gualtar, 4710-057, Braga, Portugal.
Artur Cavaco-PauloCentre of Biological Engineering (CEB), University of Minho, Campus of Gualtar, 4710-057, Braga, Portugal.
University of Minho · PT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recent studies have demonstrated that human neutrophil elastase (HNE) can be used as marker for inflammation/infection of chronic wounds since it was found to be present in high concentration in exudate collected from chronic wounds. Biosensors used in wound care benefit from a chromogenic signalling due to the readiness of signal interpretation, but the most common use faint yellow chromogenic molecules such as

Indexed as

Chromogenic GFP-like proteinsE11, 11th β-strand of ultramarine inserted in a eglin c portion with an HNE cleavage sequenceHNE, human neutrophil elastaseHuman neutrophil elastase (HNE)Molecular dynamics simulationspep11, synthetic peptide equivalent to free 11th β-sheet of ultramarinepNa, p-nitroanilineProteasesRMSF, root-mean-square fluctuationSplit GFPUltramarine (UM)UM10, ultramarine engineered without the last 11th β-strandUM, ultramarine protein

Identifiers

PMID31049301
PMCPMC6479270
OpenAlexW2922167189

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.