ArticleJournal of biological engineering2019
krCRISPR: an easy and efficient strategy for generating conditional knockout of essential genes in cells.
Article in Journal of biological engineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 37 citations in OpenAlex.
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- Phage-assisted evolution of compact Cas9 variants targeting a simple NNG PAM.Nature chemical biology · 2024Article
- Prediction of base editor off-targets by deep learning.Nature communications · 2023Article
- Genome editing with natural and engineered CjCas9 orthologs.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Identification of SaCas9 orthologs containing a conserved serine residue that determines simple NNGG PAM recognition.PLoS biology · 2022Article
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- Compact SchCas9 Recognizes the Simple NNGR PAM.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A Highly Sensitive GFP Activation Assay for Detection of DNA Cleavage in Cells.Frontiers in cell and developmental biology · 2021Article
- A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope.PLoS biology · 2020Article
- Characterization ofFrontiers in cell and developmental biology · 2020Article
- Base Editing Mediated Generation of Point Mutations Into Human Pluripotent Stem Cells for Modeling Disease.Frontiers in cell and developmental biology · 2020Article
- Bioengineering tools for the production of pharmaceuticals: current perspective and future outlook.Bioengineered · 2019Review
- Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCRISPR/Cas9 system is a powerful tool for knocking out genes in cells. However, genes essential for cell survival cannot be directly knocked out. Traditionally, generation of conditional knockout cells requires multiple steps.
resultsIn this study, we developed an easy and efficient strategy to generate conditional knockout cells by using double episomal vectors - one which expresses gRNA and Cas9 nuclease, and the other expresses an inducible rescue gene. Using this system which we named "krCRISPR" (knockout-rescue CRISPR), we showed that essential genes,
conclusionsThe krCRISPR system offers an easy and efficient platform that facilitates the study of essential genes' function.
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Registered trials
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