Evidence map›Paper›PMID 31048082›Full record

ArticleNanomedicine : nanotechnology, biology, and medicine2019

Subtle changes in surface-tethered groups on PEGylated DNA nanoparticles significantly influence gene transfection and cellular uptake.

Xiyu Ke, Zonghui Wei, Ying Wang, Sabrina Shen, Yong Ren, John-Michael Williford, Erik Luijten, Hai-Quan Mao

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nanomedicine : nanotechnology, biology, and medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Xiyu KeDepartment of Materials Science and Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, United States; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, United States.
Zonghui WeiGraduate Program in Applied Physics, Northwestern University, Evanston, IL, United States.
Ying WangDepartment of Materials Science and Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, United States; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, United States.
Sabrina ShenDepartment of Materials Science and Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, United States.
Yong RenDepartment of Materials Science and Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, United States; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, United States.
John-Michael WillifordInstitute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, United States.
Erik LuijtenDepartment of Materials Science and Engineering, Northwestern University, Evanston, IL, United States; Department of Engineering Sciences and Applied Mathematics, Northwestern University, Evanston, IL, United States; Department of Physics and Astronomy, Northwestern University, Evanston, IL, United States. Electronic address: luijten@northwestern.edu.
Hai-Quan MaoDepartment of Materials Science and Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, United States; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, United States; Translational Tissue Engineering Center and Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD, United States. Electronic address: hmao@jhu.edu.
Johns Hopkins University · USNorthwestern University · USJohns Hopkins Medicine · US

Funding

Shape Control and Transport Properties of DNA-Copolymer MicellesR01EB018358 · NIBIB · JOHNS HOPKINS UNIVERSITY · PI LUIJTEN, ERIK, MAO, HAI-QUAN · 2015 to 2018
$2.1M
NIBIB NIH HHS R01 EB018358
6 · The paper itself

Abstract

PEGylation strategy has been widely used to enhance colloidal stability of polycation/DNA nanoparticles (NPs) for gene delivery. To investigate the effect of polyethylene glycol (PEG) terminal groups on the transfection properties of these NPs, we synthesized DNA NPs using PEG-g-linear polyethyleneimine (lPEI) with PEG terminal groups containing alkyl chains of various lengths with or without a hydroxyl terminal group. For both alkyl- and hydroxyalkyl-decorated NPs with PEG grafting densities of 1.5, 3, or 5% on lPEI, the highest levels of transfection and uptake were consistently achieved at intermediate alkyl chain lengths of 3 to 6 carbons, where the transfection efficiency is significantly higher than that of nonfunctionalized lPEI/DNA NPs. Molecular dynamics simulations revealed that both alkyl- and hydroxyalkyl-decorated NPs with intermediate alkyl chain length exhibited more rapid engulfment than NPs with shorter or longer alkyl chains. This study identifies a new parameter for the engineering design of PEGylated DNA NPs.

Indexed as

EndocytosisTransfectionCell Line, TumorDNAHumansHydrophobic and Hydrophilic InteractionsLipid BilayersMolecular Dynamics SimulationNanoparticlesPolyethylene GlycolsDNALipid BilayersPolyethylene GlycolsCellular uptakeMolecular dynamics simulationPEG terminal groupPEGylated DNA nanoparticlesTransfection

Identifiers

PMID31048082
PMCPMC7386071
OpenAlexW2942901994

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.