ArticleJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2019
Sensitive detection of porcine circovirus 3 by droplet digital PCR.
Article in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Advancements in Modern Nucleic Acid-Based Multiplex Testing Methodologies for the Diagnosis of Swine Infectious Diseases.Veterinary sciences · 2025Review
- Characterization of ovine gammaherpesvirus 2 in a goat by nanoplate digital PCR and other diagnostic methods.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Development and clinical validation of a dual ddPCR assay for detecting carbapenem-resistantFrontiers in microbiology · 2024Article
- Development of a droplet digital PCR method for detection of porcine circovirus 4.BMC veterinary research · 2023Article
- Revisiting Porcine Circovirus Infection: Recent Insights and Its Significance in the Piggery Sector.Vaccines · 2023Review
- Colorimetric Kit for Rapid Porcine Circovirus 2 (PCV-2) Diagnosis.Pathogens (Basel, Switzerland) · 2022Article
- Single Multiple Cross Displacement Amplification for Rapid and Real-Time Detection of Porcine Circovirus 3.Frontiers in veterinary science · 2021Article
- Simultaneous Detection and Viral DNA Load Quantification of Different Human Papillomavirus Types in Clinical Specimens by the High Analytical Droplet Digital PCR Method.Frontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Porcine circovirus 3 (PCV-3) is a newly emerging virus that poses a potential threat to the swine industry. We developed a sensitive assay utilizing droplet digital PCR (ddPCR) to detect PCV-3. Specificity of the assay was confirmed by the failure of amplification of DNA of other relevant viruses. The detection limit for ddPCR was 1 copy/μL, 10 times greater sensitivity than TaqMan real-time PCR (rtPCR). Both methods showed a high degree of linearity, although TaqMan rtPCR showed less sensitivity than ddPCR for clinical detection. Our findings indicate that ddPCR might offer faster and improved analytical sensitivity for PCV-3 detection.
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