ArticleAnalytical chemistry2019
Interlaboratory Comparison of Hydrogen-Deuterium Exchange Mass Spectrometry Measurements of the Fab Fragment of NISTmAb.
Open access · greenAbstract readComparative Study
In one paragraph
Article in Analytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24citing papers in PubMed
4.7field-weighted citation impact, top 4% of its field
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
- Nanoscaled, Fully Automated Hydrogen/Deuterium Exchange for Analysis of Macromolecular Assemblies Reaching the MDa Scale.Molecular & cellular proteomics : MCP · 2026Article
- NIST Biomanufacturing Reference Materials: Development, Applications, and Impact.Bridge (Washington, D.C. : 1969) · 2025Article
- Rapid Removal of IgG1 Carryover on Protease Column Using Protease-Safe Wash Solutions Delivered with LC Pump for HDX-MS Systems.Journal of the American Society for Mass Spectrometry · 2025Article
- Hydrophilic Interaction Liquid Chromatography at Subzero Temperature for Hydrogen-Deuterium Exchange Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2023Article
- Article
- Probing Antibody Structures by Hydrogen/Deuterium Exchange Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Chromatography at -30 °C for Reduced Back-Exchange, Reduced Carryover, and Improved Dynamic Range for Hydrogen-Deuterium Exchange Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2022Article
- Epitope mapping of antibodies in C-reactive protein assay kits by hydrogen-deuterium exchange mass spectrometry explains differential results across kits.Analytical and bioanalytical chemistry · 2022Article
- Advances in Hydrogen/Deuterium Exchange Mass Spectrometry and the Pursuit of Challenging Biological Systems.Chemical reviews · 2022Review
- Computational Structure Prediction for Antibody-Antigen Complexes From Hydrogen-Deuterium Exchange Mass Spectrometry: Challenges and Outlook.Frontiers in immunology · 2022Review
- Interlaboratory Studies Using the NISTmAb to Advance Biopharmaceutical Structural Analytics.Frontiers in molecular biosciences · 2022Review
- Analytical Similarity Assessment of Biosimilars: Global Regulatory Landscape, Recent Studies and Major Advancements in Orthogonal Platforms.Frontiers in bioengineering and biotechnology · 2022Review
- Simple Platform for Automating Decoupled LC-MS Analysis of Hydrogen/Deuterium Exchange Samples.Journal of the American Society for Mass Spectrometry · 2021Article
- Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the FcγRIa (CD64) Receptor.Journal of research of the National Institute of Standards and Technology · 2021Article
- Hydrogen-Deuterium Exchange Mass Spectrometry: A Novel Structural Biology Approach to Structure, Dynamics and Interactions of Proteins and Their Complexes.Life (Basel, Switzerland) · 2020Review
- Conformational gating, dynamics and allostery in human monoacylglycerol lipase.Scientific reports · 2020Article
- Imidazolium Compounds as Internal Exchange Reporters for Hydrogen/Deuterium Exchange by Mass Spectrometry.Analytical chemistry · 2020Article
- HDX-MS: An Analytical Tool to Capture Protein Motion in Action.Biomedicines · 2020Review
- Covalent Labeling/Mass Spectrometry of Monoclonal Antibodies with Diethylpyrocarbonate: Reaction Kinetics for Ensuring Protein Structural Integrity.Journal of the American Society for Mass Spectrometry · 2020Article
- Interpretation of HDX Data by Maximum-Entropy Reweighting of Simulated Structural Ensembles.Biophysical journal · 2020Article
Corrections and comments
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Authors and funding
37 authors at 12 institutions in 4 countries.
Jeffrey W HudgensBioprocess Measurement Group, Biomolecular Measurements Division , National Institute of Standards and Technology , Rockville , Maryland 20850 , United States.ORCID 0000-0003-2805-1048
Elyssia S GallagherBioprocess Measurement Group, Biomolecular Measurements Division , National Institute of Standards and Technology , Rockville , Maryland 20850 , United States.ORCID 0000-0002-5411-7285
Ioannis KarageorgosBioprocess Measurement Group, Biomolecular Measurements Division , National Institute of Standards and Technology , Rockville , Maryland 20850 , United States.ORCID 0000-0002-2799-766X
Kyle W AndersonBioprocess Measurement Group, Biomolecular Measurements Division , National Institute of Standards and Technology , Rockville , Maryland 20850 , United States.ORCID 0000-0002-2808-3026
James J FillibenStatistical Engineering Division , National Institute of Standards and Technology , Gaithersburg , Maryland 20899 , United States.ORCID 0000-0002-2388-3198
Richard Y-C HuangPharmaceutical Candidate Optimization, Research and Development , Bristol-Myers Squibb Company , Princeton , New Jersey 08540 , United States.ORCID 0000-0002-7172-110X
Guodong ChenPharmaceutical Candidate Optimization, Research and Development , Bristol-Myers Squibb Company , Princeton , New Jersey 08540 , United States.ORCID 0000-0002-5531-407X
George M Bou-AssafAnalytical Development , Biogen Inc. , 225 Binney Street , Cambridge , Massachusetts 02142 , United States.ORCID 0000-0003-4285-5915
Alfonso EspadaCentro de Investigación Lilly S.A. , 28108 Alcobendas , Spain.ORCID 0000-0002-8301-1201
Michael J ChalmersLilly Research Laboratories , Eli Lilly and Company , Indianapolis , Indiana 46285 , United States.ORCID 0000-0002-2139-6089
Eduardo HarguindeyCentro de Investigación Lilly S.A. , 28108 Alcobendas , Spain.ORCID 0000-0002-9291-0591
Hui-Min ZhangProtein Analytical Chemistry , Genentech, Inc. , 1 DNA Way , South San Francisco , California 94080 , United States.ORCID 0000-0002-2099-4730
Benjamin T WaltersProtein Analytical Chemistry , Genentech, Inc. , 1 DNA Way , South San Francisco , California 94080 , United States.ORCID 0000-0001-5400-0696
Jennifer ZhangProtein Analytical Chemistry , Genentech, Inc. , 1 DNA Way , South San Francisco , California 94080 , United States.ORCID 0000-0002-9036-0477
John VenableGenomics Institute of the Novartis Research Foundation , 10675 John Jay Hopkins Drive , San Diego , California 92121 , United States.ORCID 0000-0002-9938-4643
Caitlin StecklerGenomics Institute of the Novartis Research Foundation , 10675 John Jay Hopkins Drive , San Diego , California 92121 , United States.
Inhee ParkGenomics Institute of the Novartis Research Foundation , 10675 John Jay Hopkins Drive , San Diego , California 92121 , United States.ORCID 0000-0001-9161-438X
Ansgar BrockGenomics Institute of the Novartis Research Foundation , 10675 John Jay Hopkins Drive , San Diego , California 92121 , United States.ORCID 0000-0002-8080-3107
Xiaojun LuMedImmune LLC , One MedImmune Way , Gaithersburg , Maryland 20878 , United States.ORCID 0000-0001-5251-4343
Ratnesh PandeyMedImmune LLC , One MedImmune Way , Gaithersburg , Maryland 20878 , United States.ORCID 0000-0002-0072-6480
Arun ChandramohanDepartment of Biological Sciences , National University of Singapore , 14, Science Drive 4 , Singapore 117543.ORCID 0000-0002-4175-5886
Ganesh Srinivasan AnandDepartment of Biological Sciences , National University of Singapore , 14, Science Drive 4 , Singapore 117543.ORCID 0000-0001-8995-3067
Sasidhar N NirudodhiVaccine R&D , Pfizer Inc. , 401 N Middletown Rd , Pearl River, New York 10965 , United States.ORCID 0000-0002-1827-7177
Justin B SperryAnalytical R&D , Pfizer Inc. , 700 Chesterfield Parkway West , Chesterfield , Missouri 63017 , United States.ORCID 0000-0002-3274-2194
Jason C RouseAnalytical R&D , Pfizer Inc. , 1 Burtt Road , Andover , Massachusetts 01810 , United States.ORCID 0000-0002-2721-7264
James A CarrollAnalytical R&D , Pfizer Inc. , 700 Chesterfield Parkway West , Chesterfield , Missouri 63017 , United States.ORCID 0000-0001-6790-0087
Kasper D RandDepartment of Pharmacy , University of Copenhagen , Universitetsparken 2 , DK-2100 Copenhagen , Denmark.ORCID 0000-0002-6337-5489
Ulrike LeursDepartment of Pharmacy , University of Copenhagen , Universitetsparken 2 , DK-2100 Copenhagen , Denmark.ORCID 0000-0002-8828-3505
David D WeisDepartment of Chemistry , University of Kansas , 1567 Irving Hill Road , Lawrence , Kansas 66045 , United States.ORCID 0000-0003-3032-1211
Mohammed A Al-NaqshabandiDepartment of Chemistry , University of Kansas , 1567 Irving Hill Road , Lawrence , Kansas 66045 , United States.ORCID 0000-0003-0839-5636
Tyler S HagemanDepartment of Chemistry , University of Kansas , 1567 Irving Hill Road , Lawrence , Kansas 66045 , United States.ORCID 0000-0003-0112-8893
Daniel DeredgeDepartment of Pharmaceutical Sciences , University of Maryland, Baltimore, School of Pharmacy , 20 North Pine Street , Baltimore , Maryland 21201 , United States.ORCID 0000-0002-6897-6523
Patrick L WintrodeDepartment of Pharmaceutical Sciences , University of Maryland, Baltimore, School of Pharmacy , 20 North Pine Street , Baltimore , Maryland 21201 , United States.ORCID 0000-0003-1866-9397
Malvina PapanastasiouDepartment of Pathology & Laboratory Medicine, Perelman School of Medicine, 402 Stellar-Chance Laboratories , University of Pennsylvania , 422 Curie Boulevard , Philadelphia , Pennsylvania 19104 , United States.ORCID 0000-0003-3378-6612
John D LambrisDepartment of Pathology & Laboratory Medicine, Perelman School of Medicine, 402 Stellar-Chance Laboratories , University of Pennsylvania , 422 Curie Boulevard , Philadelphia , Pennsylvania 19104 , United States.ORCID 0000-0002-9370-5776
Sheng LiDepartment of Medicine , University of California, San Diego , 9500 Gilman Drive , La Jolla , California 92093 , United States.ORCID 0000-0002-9073-6809
Sarah UrataDepartment of Medicine , University of California, San Diego , 9500 Gilman Drive , La Jolla , California 92093 , United States.ORCID 0000-0001-7512-4081
National Institute of Standards and Technology · USGenomics Institute of the Novartis Research Foundation · USPfizer (United States) · USUniversity of Kansas · USBristol-Myers Squibb (United States) · USNational University of Singapore · SGUniversity of California San Diego · USUniversity of Copenhagen · DKUniversity of Maryland, Baltimore · USUniversity of Pennsylvania · USBiogen (United States) · USEli Lilly (United States) · US
Funding
Structure Determination Core at SSRLU54GM094586 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI LESLEY, SCOTT ALLAN · 2010 to 2014
$40.1MRole of complement in kidney disorders: from hemodialysis to transportationP01AI068730 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI LAMBRIS, JOHN D · 2007 to 2018
$16.0MSTRUCTURE-FUNCTION ANALYSIS OF DIFFERENT C3 SPECIESR01AI030040 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI LAMBRIS, JOHN D · 1993 to 2016
$4.1MVIRAL RESPIRATORY PATHOGENS RESEARCH UNIT /VRPRU/-266030040-266030040N01AI030040 · NIAID · UNIVERSITY OF IOWA · PI APICELLA, MICHAEL A. · 2003 to 2006
–Intramural NIST DOC 9999-NISTNIAID NIH HHS N01 AI030040NIAID NIH HHS P01 AI068730NIAID NIH HHS R01 AI030040NIGMS NIH HHS U54 GM094586
Abstract
Hydrogen-deuterium exchange mass spectrometry (HDX-MS) is an established, powerful tool for investigating protein-ligand interactions, protein folding, and protein dynamics. However, HDX-MS is still an emergent tool for quality control of biopharmaceuticals and for establishing dynamic similarity between a biosimilar and an innovator therapeutic. Because industry will conduct quality control and similarity measurements over a product lifetime and in multiple locations, an understanding of HDX-MS reproducibility is critical. To determine the reproducibility of continuous-labeling, bottom-up HDX-MS measurements, the present interlaboratory comparison project evaluated deuterium uptake data from the Fab fragment of NISTmAb reference material (PDB: 5K8A ) from 15 laboratories. Laboratories reported ∼89 800 centroid measurements for 430 proteolytic peptide sequences of the Fab fragment (∼78 900 centroids), giving ∼100% coverage, and ∼10 900 centroid measurements for 77 peptide sequences of the Fc fragment. Nearly half of peptide sequences are unique to the reporting laboratory, and only two sequences are reported by all laboratories. The majority of the laboratories (87%) exhibited centroid mass laboratory repeatability precisions of ⟨ s
Indexed as
Hydrogen Deuterium Exchange-Mass SpectrometryAntibodies, MonoclonalImmunoglobulin Fab FragmentsAntibodies, MonoclonalImmunoglobulin Fab Fragments
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