ArticleApplied optics2019
Multicolor super-resolution imaging using spectroscopic single-molecule localization microscopy with optimal spectral dispersion.
Article in Applied optics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Molecular engineering of Cyanine-Hoechst hybrid materials for nanoscale DNA and chromatin imaging.JPhys materials · 2026Article
- Solvatochromic BODIPYs with Polarity-Independent Absorption for Functional Super-resolution Imaging of Live Cell Membranes.Chemical & biomedical imaging · 2026Article
- SIMBA: an agentic AI platform for single-molecule multidimensional imaging.bioRxiv : the preprint server for biology · 2026Article
- Spectroscopic DNA-PAINT for simultaneous multiplexed super-resolution microscopy.bioRxiv : the preprint server for biology · 2026Article
- Functional super-resolution microscopy of fibers and polymers: convergence of artificial and biological systems at the nanoscale.Nanoscale horizons · 2026Review
- Orthogonally Dispersed Spectroscopic Single-Molecule Localization Microscopy.Nanophotonics (Berlin, Germany) · 2026Article
- Framework for Accurate Single-Molecule Spectroscopic Imaging Analyses Using Monte Carlo Simulation and Deep Learning.Analytical chemistry · 2025Article
- DeepQR: single-molecule QR codes for optical gene-expression analysis.Nanophotonics (Berlin, Germany) · 2025Article
- Three-color single-molecule localization microscopy in chromatin.Light, science & applications · 2025Article
- Deep learning-based spectroscopic single-molecule localization microscopy.Journal of biomedical optics · 2024Article
- Blinking characteristics of organic fluorophores for blink-based multiplexing.Communications chemistry · 2024Article
- KCBC - a correlation-based method for co-localization analysis of super-resolution microscopy images using bivariate Ripley'sJournal of applied statistics · 2024Article
- Spectral precision improvement with demagnifying spectral images in spectroscopic nanoscopy.Journal of the Optical Society of America. A, Optics, image science, and vision · 2024Article
- Switchable and Functional Fluorophores for Multidimensional Single-Molecule Localization Microscopy.Chemical & biomedical imaging · 2023Review
- Spectroscopic single-molecule localization microscopy: applications and prospective.Nano convergence · 2023Review
- Minimizing Molecular Misidentification in Imaging Low-Abundance Protein Interactions Using Spectroscopic Single-Molecule Localization Microscopy.Analytical chemistry · 2022Article
- Mapping the relationship between total and functional antibodies conjugated to nanoparticles with spectrally-resolved direct stochastic optical reconstruction microscopy (SR-dSTORM).Nanoscale advances · 2022Article
- Mapping the Nicotinic Acetylcholine Receptor Nanocluster Topography at the Cell Membrane with STED and STORM Nanoscopies.International journal of molecular sciences · 2022Article
- Monolithic dual-wedge prism-based spectroscopic single-molecule localization microscopy.Nanophotonics (Berlin, Germany) · 2022Article
- Multimodal single-molecule microscopy with continuously controlled spectral resolution.Biophysical reports · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
We developed transmission diffraction grating-based spectroscopic single-molecule localization microscopy (sSMLM) to collect the spatial and spectral information of single-molecule blinking events concurrently. We characterized the spectral heterogeneities of multiple far-red emitting dyes in a high-throughput manner using sSMLM. We also investigated the influence of spectral dispersion on the single-molecule identification performance of fluorophores with large spectral overlapping. The careful tuning of spectral dispersion in grating-based sSMLM permitted simultaneous three-color super-resolution imaging in fixed cells with a single objective lens at a relatively low photon budget. Our sSMLM has a compact optical design and can be integrated with conventional localization microscopy to provide add-on spectroscopic analysis capability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.