ArticleCommunications biology2019
Evidence-based guidelines for controlling pH in mammalian live-cell culture systems.
Article in Communications biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 134 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
134 citing papers in PubMed, 248 citations in OpenAlex.
- The Effects of Extracellular Vesicles From Cancer Cells in Acidosis or ER Stress to Endothelial Cells: Angiogenesis and Endothelial Activation.Biology of the cell · 2026Article
- Protonation- and substrate-regulated dimer opening couples brain-type creatine kinase to vesicular and actin-remodeling membranes.Research square · 2026Article
- Chemistry-informed Bayesian control and policy distillation for autonomous pH adjustment in diverse chemical systems.Chemical science · 2026Article
- DYNAMEX: A Time-Resolved Model for Predicting Distribution of Chemicals in In Vitro Cell Assays.Environmental science & technology · 2026Article
- Acidic and alkaline conditions enhance Staphylococcus aureus α-toxin pore formation and cytotoxicity.PLoS pathogens · 2026Article
- pH-Dependent Silica Nanoshell Degradation Influences SERRS Enhancement in Biological Environments.ACS measurement science au · 2026Article
- ICSI media buffers: is there room for improvement?Journal of assisted reproduction and genetics · 2026Review
- Understanding Osmolality as a Critical Process Parameter in Mammalian Muscle Cell Culture and Biomanufacturing.ACS omega · 2026Review
- Multifunctional System with Ferulic Acid: Increased Safety, Antioxidant Activity, and Efficacy of Sunscreen Formulations.Antioxidants (Basel, Switzerland) · 2026Article
- IncuSense: IoT-Enabled Wireless Long-Term Multiparametric Cell Culture Incubator Monitoring System.Annals of biomedical engineering · 2026Article
- The Reactivity of Diiron(I) Bis-cyclopentadienyl Tricarbonyl Aminocarbyne Complexes in Aqueous Media: A Case Study for Iron-Based Anticancer Agents.Inorganic chemistry · 2026Article
- Stable bioreactor control reveals acidic pH-driven metabolic reprogramming and mitochondrial dysfunction in human lymphoblastoid cells.Communications biology · 2026Article
- pH-Dependent Microenvironmental Ionic Signaling in Pancreatic Ductal Adenocarcinoma.Acta physiologica (Oxford, England) · 2026Review
- Escape from cell uptake: Drug-Free cancer therapeutics regulated by hydrophobicity and negative charge.Materials today. Bio · 2026Article
- A Biomimetic Buffering Hydrogel Scaffold for Long-Term Culture of Patient-Derived Tumor Organoids.Advanced healthcare materials · 2026Article
- MicroAge mission: experimental design and hardware for a bespoke culture system supporting tissue-engineered skeletal muscle.NPJ microgravity · 2026Article
- ProlongedFrontiers in transplantation · 2026Article
- Mass Spectrometry as a Tool for Metabolomics in Cancer.Advances in experimental medicine and biology · 2026Review
- Next-generation T cell engagers for cancer and autoimmune diseases.Frontiers in immunology · 2026Review
- FAM111B Suppression Enhances Sensitivity to Gemcitabine in Pancreatic Cancer Through Intracellular pH Regulation.Cancer science · 2025Article
74 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A fundamental variable in culture medium is its pH, which must be controlled by an appropriately formulated buffering regime, since biological processes are exquisitely sensitive to acid-base chemistry. Although awareness of the importance of pH is fostered early in the training of researchers, there are no consensus guidelines for best practice in managing pH in cell cultures, and reporting standards relating to pH are typically inadequate. Furthermore, many laboratories adopt bespoke approaches to controlling pH, some of which inadvertently produce artefacts that increase noise, compromise reproducibility or lead to the misinterpretation of data. Here, we use real-time measurements of medium pH and intracellular pH under live-cell culture conditions to describe the effects of various buffering regimes, including physiological CO
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.