ArticleBiotechnology for biofuels2019
Evolutionary engineering in
Article in Biotechnology for biofuels, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 52 citations in OpenAlex.
- Establishing Saccharomyces cerevisiae as a host for renewable acrylic acid production.Microbial cell factories · 2026Article
- Yeast Stress Response to Synthetic Constructs.ACS synthetic biology · 2026Review
- Short-Chain Fatty Acid Utilization inMicroorganisms · 2025Article
- The coming wave of confluent biosynthetic, bioinformational and bioengineering technologies.Nature communications · 2025Review
- Engineering a xylose fermenting yeast for lignocellulosic ethanol production.Nature chemical biology · 2025Article
- Activation of the yeast Retrograde Response pathway by adaptive laboratory evolution with S-(2-aminoethyl)-L-cysteine reduces ethanol and increases glycerol during winemaking.Microbial cell factories · 2024Article
- Increased CONature communications · 2024Article
- The role of ion homeostasis in adaptation and tolerance to acetic acid stress in yeasts.FEMS yeast research · 2024Review
- The superior growth of Kluyveromyces marxianus at very low potassium concentrations is enabled by the high-affinity potassium transporter Hak1.FEMS yeast research · 2024Article
- General mechanisms of weak acid-tolerance and current strategies for the development of tolerant yeasts.World journal of microbiology & biotechnology · 2023Review
- The Hrk1 kinase is a determinant of acetic acid tolerance in yeast by modulating HMicrobial cell (Graz, Austria) · 2023Article
- Have you tried turning it off and on again? Oscillating selection to enhance fitness-landscape traversal in adaptive laboratory evolution experiments.Metabolic engineering communications · 2023Review
- Yeast Trk1 Potassium Transporter Gradually Changes Its Affinity in Response to Both External and Internal Signals.Journal of fungi (Basel, Switzerland) · 2022Article
- Article
- One major facilitator superfamily transporter is responsible for propionic acid tolerance in Pseudomonas putida KT2440.Microbial biotechnology · 2021Article
- Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae.Nature communications · 2020Article
- Research on the Applications of Calcium Propionate in Dairy Cows: A Review.Animals : an open access journal from MDPI · 2020Review
- Evolutionary engineering and molecular characterization of a caffeine-resistant Saccharomyces cerevisiae strain.World journal of microbiology & biotechnology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPropionic acid (PA), a key platform chemical produced as a by-product during petroleum refining, has been widely used as a food preservative and an important chemical intermediate in many industries. Microbial PA production through engineering yeast as a cell factory is a potentially sustainable alternative to replace petroleum refining. However, PA inhibits yeast growth at concentrations well below the titers typically required for a commercial bioprocess.
resultsAdaptive laboratory evolution (ALE) with PA concentrations ranging from 15 to 45 mM enabled the isolation of yeast strains with more than threefold improved tolerance to PA. Through whole genome sequencing and CRISPR-Cas9-mediated reverse engineering, unique mutations in
conclusionOur study has demonstrated the use of ALE as a powerful tool to improve yeast tolerance to PA. Potassium transport and maintenance is not only critical in yeast tolerance to PA but also boosts tolerance to multiple organic acids. These results demonstrate high-affinity potassium transport as a new principle for improving organic acid tolerance in strain engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.