ArticleACS synthetic biology2019
One-Pot Assembly of Complex Giant Unilamellar Vesicle-Based Synthetic Cells.
Article in ACS synthetic biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 154 citations in OpenAlex.
- Reversible Charge-Mediated Capture and Release of Extracellular Vesicles Using Giant Unilamellar Vesicles.ACS omega · 2026Article
- Environmental Impact of Synthetic Cell Technology: Review of Life Cycle Assessment Data for Feedstocks and Production Methods.Chembiochem : a European journal of chemical biology · 2026Review
- Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026Review
- Mechanically Programmable DNA Hydrogel Microparticles for 3D Cellular Systems.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- QuantGUV: Quantifying Encapsulation Efficiency of Small Molecules in Giant Unilamellar Vesicles.ACS applied materials & interfaces · 2026Article
- Stress granule phase separation in stress-responsive cytosolic extract-in-oil droplets.Nature communications · 2026Article
- Ion- and Temperature-Programmable Reconfiguration of Subcompartments in Synthetic Cells.Chembiochem : a European journal of chemical biology · 2026Article
- Mechanical performance of hybrid polymer-lipid vesicles with leaflet asymmetry engineered using microfluidics.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Self-assembled cell-scale containers made from DNA origami membranes.Nature materials · 2026Article
- Characterization and Optimization of Vesicle Properties in bioPISA: from Size Distribution to Post-Assembly Loading.Advanced biology · 2025Article
- Scaling Up Synthetic Cell Production Using Robotics and Machine Learning Toward Therapeutic Applications.Advanced biology · 2025Article
- Droplet acoustofluidics: Recent progress and challenges.Biomicrofluidics · 2025Review
- Mass-Guided Single-Cell MALDI Imaging of Low-Mass Metabolites Reveals Cellular Activation Markers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A New Means to Generate Liposomes by Rehydrating Engineered Lipid Nanoconstructs.Micromachines · 2025Article
- Internal State of Vesicles Affects Higher Order State of Vesicle Assembly and Interaction.ACS omega · 2024Article
- DNA microbeads for spatio-temporally controlled morphogen release within organoids.Nature nanotechnology · 2024Article
- Article
- Biomimetic Nanoparticles for Basic Drug Delivery.Pharmaceutics · 2024Review
- A synthetic method to assay polycystin channel biophysics.bioRxiv : the preprint server for biology · 2024Article
- Artificial cells for in vivo biomedical applications through red blood cell biomimicry.Nature communications · 2024Review
2 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we introduce a one-pot method for the bottom-up assembly of complex single- and multicompartment synthetic cells. Cellular components are enclosed within giant unilamellar vesicles (GUVs), produced at the milliliter scale directly from small unilamellar vesicles (SUVs) or proteoliposomes with only basic laboratory equipment within minutes. Toward this end, we layer an aqueous solution, containing SUVs and all biocomponents, on top of an oil-surfactant mix. Manual shaking induces the spontaneous formation of surfactant-stabilized water-in-oil droplets with a spherical supported lipid bilayer at their periphery. Finally, to release GUV-based synthetic cells from the oil and the surfactant shell into the physiological environment, we add an aqueous buffer and a droplet-destabilizing agent. We prove that the obtained GUVs are unilamellar by reconstituting the pore-forming membrane protein α-hemolysin and assess the membrane quality with cryotransmission electron microscopy (cryoTEM), fluorescence recovery after photobleaching (FRAP), and zeta-potential measurements as well as confocal fluorescence imaging. We further demonstrate that our GUV formation method overcomes key challenges of standard techniques, offering high volumes, a flexible choice of lipid compositions and buffer conditions, straightforward coreconstitution of proteins, and a high encapsulation efficiency of biomolecules and even large cargo including cells. We thereby provide a simple, robust, and broadly applicable strategy to mass-produce complex multicomponent GUVs for high-throughput testing in synthetic biology and biomedicine, which can directly be implemented in laboratories around the world.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.