Evidence map›Paper›PMID 31041748›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Analysis of the Transcriptional Activity of Retinoic Acid-Related Orphan Receptors (RORs) and Inhibition by Inverse Agonists.

Yukimasa Takeda, Hong Soon Kang, Anton M Jetten

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Yukimasa TakedaNational Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA.
Hong Soon KangNational Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA.
Anton M JettenNational Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA. jetten@niehs.nih.gov.
National Institute of Environmental Health Sciences · USNational Institutes of Health · US

Funding

Nuclear receptors: action, functions, and roles in diseaseZIAES101586 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI JETTEN, ANTON M · 2009 to 2025
$13.5M
Nuclear receptors--Action, functions, &roles in diseaseZ01ES101586 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI JETTEN, ANTON M · 2003 to 2008
$3.2M
Intramural NIH HHS Z01 ES101586
6 · The paper itself

Abstract

Here, we describe several assays to analyze the transcriptional activity of retinoic acid-related orphan receptors (RORs) and the effect of inverse agonists on their activity. One assay measures the effect of an inverse agonist on the transcriptional activation of a luciferase reporter by RORs in a Tet-On cell system. A mammalian two-hybrid assay analyzes the interaction of the ROR ligand binding domain with a coactivator peptide. Two additional assays examine the effect of an inverse agonist on the activation of a luciferase reporter under control of the promoter of the ROR target gene, IL17, and on ROR-mediated activation using a mammalian monohybrid assay.

Indexed as

Genes, ReporterTranscriptional ActivationAnimalsBiological AssayCHO CellsCricetulusReceptors, Retinoic AcidTretinoinReceptors, Retinoic AcidTretinoin(Inverse) agonistLuciferase reporterMammalian two-hybrid and monohybrid assaysPromoterReporter assaysRetinoic acid-related orphan receptorRORαRORγTranscriptional activation

Identifiers

PMID31041748
PMCPMC6785025
OpenAlexW2943266026

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.