ArticleScientific reports2019
An HRM Assay to Differentiate Sheeppox Virus Vaccine Strains from Sheeppox Virus Field Isolates and other Capripoxvirus Species.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Molecular detection and isolation of Lumpy Skin Disease Virus from outbreak cases in Ilubabor Zone, Oromia, Ethiopia.BMC microbiology · 2026Article
- Progress in diagnostic methods and vaccines for lumpy skin disease virus: a path towards understanding the disease.Veterinary research communications · 2025Review
- Development of a Multi-Locus Real-Time PCR with a High-Resolution Melting Assay to Differentiate Wild-Type, Asian Recombinant, and Vaccine Strains of Lumpy Skin Disease Virus.Veterinary sciences · 2025Article
- Live attenuated goatpox vaccination in pregnant Murcia-Granada goats: dosage implications and outcomes.BMC veterinary research · 2024Article
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- A novel triplex real-time PCR assay for the differentiation of lumpy skin disease virus, goatpox virus, and sheeppox virus.Frontiers in veterinary science · 2023Article
- Development and Optimization of Indirect ELISAs for the Detection of Anti-Capripoxvirus Antibodies in Cattle, Sheep, and Goat Sera.Microorganisms · 2022Article
- First Report of Lumpy Skin Disease in Myanmar and Molecular Analysis of the Field Virus Isolates.Microorganisms · 2022Article
- A robust, cost-effective and widely applicable whole-genome sequencing protocol for capripoxviruses.Journal of virological methods · 2022Article
- A high-resolution melting assay to differentiate a peste des petits ruminants virus vaccine strain from field isolates in Turkey.Archives of virology · 2022Article
- Molecular Characterization of the 2020 Outbreak of Lumpy Skin Disease in Nepal.Microorganisms · 2022Article
- High-resolution melting analysis identifies reservoir hosts of zoonotic Leishmania parasites in Tunisia.Parasites & vectors · 2022Article
- Rapid detection of Mycoplasma mycoides subsp. capri and Mycoplasma capricolum subsp. capripneumoniae using high-resolution melting curve analysis.Scientific reports · 2021Article
- Molecular Analysis of East African Lumpy Skin Disease Viruses Reveals a Mixed Isolate with Features of Both Vaccine and Field Isolates.Microorganisms · 2021Article
- Capripoxvirus Infections in Ruminants: A Review.Microorganisms · 2021Review
- Probe-Based Real-Time qPCR Assays for a Reliable Differentiation of Capripox Virus Species.Microorganisms · 2021Article
- Experimental infection of indigenous North African goats with goatpox virus.Acta veterinaria Scandinavica · 2021Article
- Review of sheep and goat pox disease: current updates on epidemiology, diagnosis, prevention and control measures in Ethiopia.Animal diseases · 2021Review
- Evaluation of Serological Tests for Detection of Antibodies against Lumpy Skin Disease Virus.Journal of clinical microbiology · 2020Article
Corrections and comments
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Authors and funding
14 authors at 7 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sheep poxvirus (SPPV), goat poxvirus (GTPV) and lumpy skin disease virus (LSDV) affect small ruminants and cattle causing sheeppox (SPP), goatpox (GTP) and lumpy skin disease (LSD) respectively. In endemic areas, vaccination with live attenuated vaccines derived from SPPV, GTPV or LSDV provides protection from SPP and GTP. As live poxviruses may cause adverse reactions in vaccinated animals, it is imperative to develop new diagnostic tools for the differentiation of SPPV field strains from attenuated vaccine strains. Within the capripoxvirus (CaPV) homolog of the variola virus B22R gene, we identified a unique region in SPPV vaccines with two deletions of 21 and 27 nucleotides and developed a High-Resolution Melting (HRM)-based assay. The HRM assay produces four distinct melting peaks, enabling the differentiation between SPPV vaccines, SPPV field isolates, GTPV and LSDV. This HRM assay is sensitive, specific, and provides a cost-effective means for the detection and classification of CaPVs and the differentiation of SPPV vaccines from SPPV field isolates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.