ArticleScientific reports2019
Development of a novel cultivation technique for uncultured soil bacteria.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
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Who cites it
83 citing papers in PubMed.
- Impact of extended fertilization on soil microbial diversity: a comparative full-length 16S rRNA amplicon analysis.Genomics & informatics · 2026Article
- Challenges and Future Directives of Synthetic Biology in Engineering Plant-Microbe Partnerships for Sustainable Agriculture.Biotechnology and bioengineering · 2026Review
- Sulfur Oxidation by New and Non-Canonical Bacteria in a Subsurface Flow Constructed Wetland Treating Domestic Wastewater.Microorganisms · 2026Article
- Primer choice shapes microbial community interpretation across habitats and informs short-term structured enrichment in environmental and applied systems.Frontiers in microbiology · 2026Article
- Study on disinfection using wet wipes and UV irradiation for areas prone to condensate accumulation in the Chinese Space Station.Frontiers in microbiology · 2026Article
- An enhanced domestication method for uncultured bacteria.ISME communications · 2026Article
- The prevalence and distribution ofFrontiers in microbiomes · 2026Article
- Fosfomycin Resistance: An Update on the Anthropogenic Impact Through Agriculture.Pathogens (Basel, Switzerland) · 2025Review
- Genome sequence of Gaetbulibacter sp. PBL-D1 isolated from a Malaysian beach: insights into glycoside hydrolase repertoires.BMC genomic data · 2025Article
- Antibiotic Resistance Crisis: From Bacterial Bioprospecting to Artificial Intelligence.Environmental microbiology reports · 2025Review
- Bacterial Proteomics and Antibiotic Resistance Identification: Is Single-Cell Analysis a Worthwhile Pursuit?Pathogens (Basel, Switzerland) · 2025Review
- Mining Microbial Dark Matter: Advanced Cultivation Techniques for Bioactive Compound Discovery.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Draft genome sequence ofMicrobiology resource announcements · 2025Article
- A droplet microfluidic strategy for cultivation, investigation, and high-throughput isolation of mouse gut microbiome bacteria.Applied and environmental microbiology · 2025Article
- A diffusion-based integrative approach for culturing previously uncultured bacteria from marine sediments.Marine life science & technology · 2025Article
- Microfluidic droplets with amended culture media cultivate a greater diversity of soil microorganisms.Applied and environmental microbiology · 2025Article
- A climatically significant abiotic mechanism driving carbon loss and nitrogen limitation in peat bogs.Scientific reports · 2025Article
- Environmental community transcriptomics: strategies and struggles.Briefings in functional genomics · 2025Review
- Harnessing iChip technology for novel antibiotic discovery from peat soil microbiomes to combat antimicrobial resistance.Frontiers in microbiology · 2025Article
- Metagenome Analysis Identified Novel Microbial Diversity of Sandy Soils Surrounded by Natural Lakes and Artificial Water Points in King Salman Bin Abdulaziz Royal Natural Reserve, Saudi Arabia.Life (Basel, Switzerland) · 2024Article
23 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
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Abstract
In this study, a new diffusion bioreactor was developed to cultivate hidden bacterial communities in their natural environment. The newly developed method was investigated to cultivate microbial communities from the forest soil, and the results were evaluated against traditional culture methods and compared to the results of a pyrosequencing-based molecular survey. The molecular analysis revealed that a diverse bacterial population was present in the soil sample. However, both the newly developed method and the traditional method recovered more than 400 isolates, which belonged to only four phyla: Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. Although these isolates were distributed over only four major phyla, the use of the newly developed technique resulted in the successful cultivation of 35 previously uncultured strains, whereas no such strains were successfully cultivated by the traditional method. Furthermore, the study also found that the recovery of uncultured bacteria and novel isolates was related to sampling season, incubation period, and cultivation media. The use of soil collected in summer, a prolonged incubation period, and low-substrate modified media increased the recovery of uncultured and novel isolates. Overall, the results indicate that the newly designed diffusion bioreactor can mimic the natural environment, which permits the cultivation of previously uncultured bacteria.
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