ArticleScientific reports2019
CRISPR-Cas9 Editing in Maize: Systematic Evaluation of Off-target Activity and Its Relevance in Crop Improvement.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Single-molecule DNA flow-stretch assays for high-throughput DNA-protein interaction studies.FEBS open bio · 2026Article
- Impact of wheat GRF4-GIF1 morphogenic regulators on transformation and genome editing efficiency in elite barley cultivars.Frontiers in plant science · 2026Article
- Off-target detection of CRISPR-Cas9 nucleaseSynthetic biology (Oxford, England) · 2026Article
- Review
- Editing theInternational journal of molecular sciences · 2025Article
- The extent of multiallelic, co-editing of LIGULELESS1 in highly polyploid sugarcane tunes leaf inclination angle and enables selection of the ideotype for biomass yield.Plant biotechnology journal · 2024Article
- Impact of predictive selection of LbCas12a CRISPR RNAs upon on- and off-target editing rates in soybean.Plant direct · 2024Article
- CRISPR/Cas9 as a Mutagenic Factor.International journal of molecular sciences · 2024Review
- Breeding and biotechnology approaches to enhance the nutritional quality of rapeseed byproducts for sustainable alternative protein sources- a critical review.Frontiers in plant science · 2024Review
- Rare but diverse off-target and somatic mutations found in field and greenhouse grown trees expressing CRISPR/Cas9.Frontiers in bioengineering and biotechnology · 2024Article
- CRISPR-Cas technology secures sustainability through its applications: a review in green biotechnology.3 Biotech · 2023Review
- The potential of metabolomics in assessing global compositional changes resulting from the application of CRISPR/Cas9 technologies.Transgenic research · 2023Article
- High-throughput sgRNA testing reveals rules for Cas9 specificity and DNA repair in tomato cells.Frontiers in genome editing · 2023Article
- Enabling Trade in Gene-Edited Produce in Asia and Australasia: The Developing Regulatory Landscape and Future Perspectives.Plants (Basel, Switzerland) · 2022Review
- Green Revolution to Gene Revolution: Technological Advances in Agriculture to Feed the World.Plants (Basel, Switzerland) · 2022Review
- Occurrence and Nature of Off-Target Modifications by CRISPR-Cas Genome Editing in Plants.ACS agricultural science & technology · 2022Review
- Optimized Transformation and Gene Editing of the B104 Public Maize Inbred by Improved Tissue Culture and Use of Morphogenic Regulators.Frontiers in plant science · 2022Article
- CRISPR-Cas technology opens a new era for the creation of novel maize germplasms.Frontiers in plant science · 2022Review
- CRISPR/Cas- and Topical RNAi-Based Technologies for Crop Management and Improvement: Reviewing the Risk Assessment and Challenges Towards a More Sustainable Agriculture.Frontiers in bioengineering and biotechnology · 2022Review
- CRISPR/Cas9 suppression of OsAT10, a rice BAHD acyltransferase, reduces p-coumaric acid incorporation into arabinoxylan without increasing saccharification.Frontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
18 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR-Cas9 enabled genome engineering has great potential for improving agriculture productivity, but the possibility of unintended off-target edits has evoked some concerns. Here we employ a three-step strategy to investigate Cas9 nuclease specificity in a complex plant genome. Our approach pairs computational prediction with genome-wide biochemical off-target detection followed by validation in maize plants. Our results reveal high frequency (up to 90%) on-target editing with no evidence of off-target cleavage activity when guide RNAs were bioinformatically predicted to be specific. Predictable off-target edits were observed but only with a promiscuous guide RNA intentionally designed to validate our approach. Off-target editing can be minimized by designing guide RNAs that are different from other genomic locations by at least three mismatches in combination with at least one mismatch occurring in the PAM proximal region. With well-designed guides, genetic variation from Cas9 off-target cleavage in plants is negligible, and much less than inherent variation.
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