ArticleScientific reports2019
Single molecule super-resolution imaging of bacterial cell pole proteins with high-throughput quantitative analysis pipeline.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Functional characterization of the polar organizer protein FimV inJournal of bacteriology · 2026Article
- Looking at bacterial cell poles from a liquid-liquid phase separation of intrinsically disordered proteins perspective.Current research in structural biology · 2025Article
- Superresolution imaging of antibiotic-induced structural disruption of bacteria enabled by photochromic glycomicelles.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Molecular mapping of neuronal architecture using STORM microscopy and new fluorescent probes for SMLM imaging.Neurophotonics · 2024Article
- Similarly slow diffusion of BAM and SecYEG complexes in live E. coli cells observed with 3D spt-PALM.Biophysical journal · 2023Article
- Microfluidic Single-Cell Analytics.Advances in biochemical engineering/biotechnology · 2022Article
- Analysis of HubP-dependent cell pole protein targeting in Vibrio cholerae uncovers novel motility regulators.PLoS genetics · 2022Article
- Computerized fluorescence microscopy of microbial cells.World journal of microbiology & biotechnology · 2021Review
- RNA-mediated control of cell shape modulates antibiotic resistance in Vibrio cholerae.Nature communications · 2020Article
- Enzyme-targeted fluorescent small-molecule probes for bacterial imaging.Current opinion in chemical biology · 2020Review
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3 authors.
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Abstract
Bacteria show sophisticated control of their cellular organization, and many bacteria deploy different polar landmark proteins to organize the cell pole. Super-resolution microscopy, such as Photo-Activated Localization Microscopy (PALM), provides the nanoscale localization of molecules and is crucial for better understanding of organization and dynamics in single-molecule. However, analytical tools are not fully available yet, in particular for bacterial cell biology. For example, quantitative and statistical analyses of subcellular localization with multiple cells from multiple fields of view are lacking. Furthermore, brightfield images are not sufficient to get accurate contours of small and low contrast bacterial cells, compared to subpixel presentation of target molecules. Here we describe a novel analytic tool for PALM which integrates precisely drawn cell outlines, of either inner membrane or periplasm, labelled by PALM-compatible fluorescent protein fusions, with molecule data for >10,000 molecules from >100 cells by fitting each cell into an oval arc. In the vibrioid bacterium Vibrio cholerae, the polar anchor HubP constitutes a big polar complex which includes multiple proteins involved in chemotaxis and the flagellum. With this pipeline, HubP is shown to be slightly skewed towards the inner curvature side of the cell, while its interaction partners showed rather loose polar localization.
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