ArticleNature communications2019
4-Coumarate 3-hydroxylase in the lignin biosynthesis pathway is a cytosolic ascorbate peroxidase.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 131 papers, 1 of them a synthesis that pooled it.
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Who cites it
131 citing papers in PubMed, 1 synthesis or guideline pooled it, 278 citations in OpenAlex.
- Origin and Evolution of Enzymes with MIO Prosthetic Group: Microbial Coevolution After the Mass Extinction Event.Frontiers in genetics · 2022Pooled it
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- Expanding agroinfiltration host range with broad-spectrum effectors monitored by autoluminescent reporter.Horticulture research · 2026Article
- GhCOR27 confers Verticillium wilt resistance in cotton by relieving the GhASIL1-GhERF114-mediated repression of lignin accumulation.Plant physiology · 2026Article
- Conserved 4-coumarate 3-hydroxylase/ascorbate peroxidase bifunctionality coordinates lignin deposition and plant growth in Brachypodium and Populus.Plant physiology · 2026Article
- Gap Analysis of Metabolic Conversions of Off-Flavors and Antinutrients in Plant-Based Substrates.Comprehensive reviews in food science and food safety · 2026Review
- Precursor-Dependent Routing of Aromatic Amino Acids Determines Lignin Structure in Grasses by Sensitivity-Enhanced Solid-State NMR.Journal of the American Chemical Society · 2026Article
- Functional characterization of a novelApplied and environmental microbiology · 2026Article
- Precursor-Dependent Routing of Aromatic Amino Acids Determines Lignin Structure in Grasses by Sensitivity-Enhanced Solid-State NMR.bioRxiv : the preprint server for biology · 2026Article
- Variations in carbon flux allocation among cassava (Manihot esculenta) cultivars arise from balanced competition between starch accumulation and structural component development.Communications biology · 2026Article
- Early occurrence of caffeoylshikimic acid esterase (CSE) activity in the hornwort Anthoceros agrestis.Planta · 2026Article
- Spicy genes: mapping quantitative genomic regions and candidate genes for capsaicinoid and capsinoid biosynthesis in pepper.Frontiers in plant science · 2026Review
- Article
- Recent Advances in Biosynthesis and Bioactivity of Plant Caffeoylquinic Acids.Current issues in molecular biology · 2025Review
- Potential of Orally Administered Quercetin, Hesperidin, andInternational journal of molecular sciences · 2025Review
- A Role for the Plastidial GPT2 Translocator in the Modulation of Lignin Biosynthesis.Plant, cell & environment · 2025Article
- Article
- Factors underlying a latitudinal gradient in the S/G lignin monomer ratio in natural poplar variants.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Haplotype-resolved, gap-free genome assemblies provide insights into the divergence between Asian and European pears.Nature genetics · 2025Article
- Nature's laboratory: plant metabolic engineering methods using phenylpropanoids as a case study.Biotechnology for biofuels and bioproducts · 2025Review
71 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 2 institutions in 1 country.
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Abstract
Lignin biosynthesis is evolutionarily conserved among higher plants and features a critical 3-hydroxylation reaction involving phenolic esters. However, increasing evidence questions the involvement of a single pathway to lignin formation in vascular plants. Here we describe an enzyme catalyzing the direct 3-hydroxylation of 4-coumarate to caffeate in lignin biosynthesis as a bifunctional peroxidase that oxidizes both ascorbate and 4-coumarate at comparable rates. A combination of biochemical and genetic evidence in the model plants Brachypodium distachyon and Arabidopsis thaliana supports a role for this coumarate 3-hydroxylase (C3H) in the early steps of lignin biosynthesis. The subsequent efficient O-methylation of caffeate to ferulate in grasses is substantiated by in vivo biochemical assays. Our results identify C3H as the only non-membrane bound hydroxylase in the lignin pathway and revise the currently accepted models of lignin biosynthesis, suggesting new gene targets to improve forage and bioenergy crops.
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