ArticleNature communications2019
Combining 3D single molecule localization strategies for reproducible bioimaging.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Lipopolysaccharide insertion and vesicle mediated turnover drive growth independent outer membrane adaptation in Escherichia coli.Nature communications · 2026Article
- Brightness demixing for simultaneous multi-target imaging in 3D single-molecule localization microscopy.Nature methods · 2026Article
- Enhanced supercritical angle localization microscopy through point spread function modeling.Biomedical optics express · 2025Article
- Single-molecule localisation microscopy approaches reveal envelope glycoprotein clusters in single-enveloped viruses: a potential functional role?Biochemical Society transactions · 2025Review
- Harnessing evanescent photoacoustic waves for multi-domain imaging.Photoacoustics · 2025Article
- Super-Resolution Goes Viral: T4 Virus Particles as Versatile 3D-Bio-NanoRulers.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Super-resolution imaging of the neuronal cytoskeleton.Npj imaging · 2024Review
- Three-Dimensional Super-Resolution Passive Cavitation Mapping in Laser Lithotripsy.IEEE transactions on ultrasonics, ferroelectrics, and frequency control · 2024Article
- Assessing crosstalk in simultaneous multicolor single-molecule localization microscopy.Cell reports methods · 2023Article
- Fibroblasts generate topographical cues that steer cancer cell migration.Science advances · 2023Article
- Global fitting for high-accuracy multi-channel single-molecule localization.Nature communications · 2022Article
- Article
- Studying the Dynamics of Chromatin-Binding Proteins in Mammalian Cells Using Single-Molecule Localization Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields.Nature communications · 2021Article
- Testing for complete spatial randomness on three dimensional bounded convex shapes.Spatial statistics · 2021Article
- Direct supercritical angle localization microscopy for nanometer 3D superresolution.Nature communications · 2021Article
- Myosin 1b flattens and prunes branched actin filaments.Journal of cell science · 2020Article
- The RNase J-Based RNA Degradosome Is Compartmentalized in the Gastric Pathogen Helicobacter pylori.mBio · 2020Article
- Defocused imaging exploits supercritical-angle fluorescence emission for precise axial single molecule localization microscopy.Biomedical optics express · 2020Article
- Carbon dots, a powerful non-toxic support for bioimaging by fluorescence nanoscopy and eradication of bacteria by photothermia.Nanoscale advances · 2019Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we present a 3D localization-based super-resolution technique providing a slowly varying localization precision over a 1 μm range with precisions down to 15 nm. The axial localization is performed through a combination of point spread function (PSF) shaping and supercritical angle fluorescence (SAF), which yields absolute axial information. Using a dual-view scheme, the axial detection is decoupled from the lateral detection and optimized independently to provide a weakly anisotropic 3D resolution over the imaging range. This method can be readily implemented on most homemade PSF shaping setups and provides drift-free, tilt-insensitive and achromatic results. Its insensitivity to these unavoidable experimental biases is especially adapted for multicolor 3D super-resolution microscopy, as we demonstrate by imaging cell cytoskeleton, living bacteria membranes and axon periodic submembrane scaffolds. We further illustrate the interest of the technique for biological multicolor imaging over a several-μm range by direct merging of multiple acquisitions at different depths.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.