Evidence map›Paper›PMID 31039819›Full record

ArticleJournal of neuroinflammation2019

Sustained interleukin-10 delivery reduces inflammation and improves motor function after spinal cord injury.

Daniel J Hellenbrand, Kaitlyn A Reichl, Benjamin J Travis, Mallory E Filipp, Andrew S Khalil, Domenic J Pulito, Ashley V Gavigan, Elizabeth R Maginot, Mitchell T Arnold, Alexander G Adler and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroinflammation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
57citing papers in PubMed, 1 pooled it
6.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

57 citing papers in PubMed, 1 synthesis or guideline pooled it, 81 citations in OpenAlex.

  1. Pooled it
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  6. Spinal Cord Injury Remyelination: Pathways to Therapies.International journal of molecular sciences · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Daniel J HellenbrandDepartment of Neurological Surgery, University of Wisconsin, Madison, WI, 53792, USA.
Kaitlyn A ReichlDepartment of Neurological Surgery, University of Wisconsin, Madison, WI, 53792, USA.
Benjamin J TravisDepartment of Neurological Surgery, University of Wisconsin, Madison, WI, 53792, USA.
Mallory E FilippDepartment of Neurological Surgery, University of Wisconsin, Madison, WI, 53792, USA.
Andrew S KhalilDepartment of Biomedical Engineering, University of Wisconsin, Madison, WI, 53706, USA.
Domenic J PulitoDepartment of Neurological Surgery, University of Wisconsin, Madison, WI, 53792, USA.
Ashley V GaviganDepartment of Neurological Surgery, University of Wisconsin, Madison, WI, 53792, USA.
Elizabeth R MaginotDepartment of Neurological Surgery, University of Wisconsin, Madison, WI, 53792, USA.
Mitchell T ArnoldDepartment of Neurological Surgery, University of Wisconsin, Madison, WI, 53792, USA.
Alexander G AdlerDepartment of Neurological Surgery, University of Wisconsin, Madison, WI, 53792, USA.
William L MurphyDepartment of Biomedical Engineering, University of Wisconsin, Madison, WI, 53706, USA.
Amgad S HannaDepartment of Neurological Surgery, University of Wisconsin, Madison, WI, 53792, USA. hanna@neurosurgery.wisc.edu.
Neurological Surgery · USUniversity of Wisconsin–Madison · US

Funding

UW COMPREHENSIVE CANCER CENTER SUPPORTP30CA014520 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI AMY TRENTHAM-DIETZ · 1985 to 2026
$142.6M
Bryon Riesch Paralysis Foundation 133-PRJ84LQFraternal Order of Eagles 133-PRJ84LQNCI NIH HHS P30 CA014520
6 · The paper itself

Abstract

backgroundThe anti-inflammatory cytokine interleukin-10 (IL-10) has been explored previously as a treatment method for spinal cord injury (SCI) due to its ability to attenuate pro-inflammatory cytokines and reduce apoptosis. Primary limitations when using systemic injections of IL-10 are that it is rapidly cleared from the injury site and that it does not cross the blood-spinal cord barrier.

objectiveHere, mineral-coated microparticles (MCMs) were used to obtain a local sustained delivery of IL-10 directly into the injury site after SCI.

methodsFemale Sprague-Dawley rats were contused at T10 and treated with either an intraperitoneal injection of IL-10, an intramedullary injection of IL-10, or MCMs bound with IL-10 (MCMs+IL-10). After treatment, cytokine levels were measured in the spinal cord, functional testing and electrophysiology were performed, axon tracers were injected into the brainstem and motor cortex, macrophage levels were counted using flow cytometry and immunohistochemistry, and lesion size was measured.

resultsWhen treated with MCMs+IL-10, IL-10 was significantly elevated in the injury site and inflammatory cytokines were significantly suppressed, prompting significantly less cells expressing antigens characteristic of inflammatory macrophages and significantly more cells expressing antigens characteristic of earlier stage anti-inflammatory macrophages. Significantly more axons were preserved within the rubrospinal and reticulospinal tracts through the injury site when treated with MCMs+IL-10; however, there was no significant difference in corticospinal tract axons preserved, regardless of treatment group. The rats treated with MCMs+IL-10 were the only group with a significantly higher functional score compared to injured controls 28 days post-contusion.

conclusionThese data demonstrate that MCMs can effectively deliver biologically active IL-10 for an extended period of time altering macrophage phenotype and aiding in functional recovery after SCI.

Indexed as

AnimalsDosage FormsFemaleInflammationInterleukin-10Neuroprotective AgentsRandom AllocationRatsRats, Sprague-DawleyRecovery of FunctionSpinal Cord InjuriesDosage FormsInterleukin-10Neuroprotective AgentsInflammationInterleukin-10MacrophagesMicrogliaSpinal cord injury

Identifiers

PMID31039819
PMCPMC6489327
OpenAlexW2947219032

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.