ArticleBiotechnology and applied biochemistry2019
Determination of parvovirus antibodies in canine serum using magnetic bead-based chemiluminescence immunoassay.
Article in Biotechnology and applied biochemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Establishment and application of a fluorescence-based nucleic acid detection system for canine parvovirus.Frontiers in veterinary science · 2025Article
- Development of a fully automated chemiluminescent immunoassay for the quantitative and qualitative detection of antibodies against African swine fever virus p72.Microbiology spectrum · 2024Article
- A chemiluminescent magnetic microparticle immunoassay for the detection of antibody against African swine fever virus.Applied microbiology and biotechnology · 2023Article
- Ultrasound-Enhanced Chemiluminescence for Bioimaging.Frontiers in bioengineering and biotechnology · 2020Review
- Pathology and molecular diagnosis of canine parvoviral enteritis in Nigeria: case report.Comparative clinical pathology · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Canine parvovirus type 2 (CPV-2), as a highly contagious and potentially fatal disease of dogs and many other carnivores, usually causes severe gastroenteritis and myocarditis. Therefore, it is very necessary and urgent to have an accurate method to determine the CPV-2 antibodies (CPV-2-Ab) in canine samples. Here, a magnetic bead-based chemiluminescence immunoassay was established and optimized to detect the concentration of CPV-2-Ab in serum. And a commercial assay was also used to evaluate the consistency with our method. After optimization of the detective system, the CPV-2-Ab was captured by CPV-antigen-magnetic bead (8.3 µg/mL); then combined with the conjugation of anti-canine IgG antibody-acridinium ester (0.36 µg/mL). Finally, collected the signal (read the luminosity) after 1 H reaction time. The linear correlation coefficient (R
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