Evidence map›Paper›PMID 31038865›Full record

ArticleInternational braz j urol : official journal of the Brazilian Society of Urology

lncRNA CCAT1 promotes bladder cancer cell proliferation, migration and invasion.

Caixiang Zhang, Wenying Wang, Jun Lin, Jing Xiao, Ye Tian

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International braz j urol : official journal of the Brazilian Society of Urology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed
4.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 84 citations in OpenAlex.

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  7. LncRNA SNHG6 role in clinicopathological parameters in cancers.European journal of medical research · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Caixiang ZhangDepartment of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Wenying WangDepartment of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Jun LinDepartment of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Jing XiaoDepartment of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Ye TianDepartment of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Capital Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo study the expression patterns of long noncoding RNA (lncRNA) colon cancer-associated transcript 1 (CCAT1) and the changes in cell proliferation, apoptosis, migration and invasion induced by silencing CCAT1 in bladder cancer cells. MATERIALS AND

methodsThe expression levels of CCAT1 were determined using realtime quantitative polymerase chain reaction in cancerous tissues and paired normal tissues from 34 patients with bladder cancer. The relationship between clinical characteristics and CCAT1 expression was analyzed. And then we conducted cell experiments. Bladder urothelial carcinoma cell lines T24 and 5637 cells were transfected with CCAT1 small interfering RNA (siRNA) or scramble siRNA. Cell proliferation and apoptosis changes were determined using a Cell Counting Kit-8 (CCK-8) assay and a fl ow cytometry assay. Migration and invasion changes were measured using a wound healing assay and a trans-well assay. microRNAs (miRNAs) were predicted by Starbase 2.0, and their differential expression levels were studied.

resultsCCAT1 was signifi cantly upregulated in bladder cancer (P < 0.05). CCAT1 upregulation was positively related to tumor stage (P = 0.004), tumor grade (P = 0.001) and tumor size (P = 0.042). Cell proliferation, migration and invasion were promoted by abnormally expressed CCAT1. miRNAs miR-181b-5p, miR-152-3p, miR-24-3p, miR-148a-3p and miR-490-3p were potentially related to the aforementioned functions of CCAT1.

conclusionCCAT1 plays an oncogenic role in urothelial carcinoma of the bladder. In addition, CCAT1 may be a potential therapeutic target in this cancer.

Indexed as

AgedCell Line, TumorCell MovementCell ProliferationDown-RegulationFemaleFlow CytometryGene ExpressionGene Expression Regulation, NeoplasticHumansMaleMicroRNAsReal-Time Polymerase Chain ReactionRNA, Long NoncodingRNA, Small InterferingTime FactorsCCAT1 long noncoding RNA, humanMicroRNAsRNA, Long NoncodingRNA, Small InterferingCCAT1 long noncoding RNA,human [Supplementary Concept]Cell ProliferationUrinary Bladder Neoplasms

Identifiers

PMID31038865
PMCPMC6786104
OpenAlexW2955177668

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.