ArticleChromosoma2019
The molecular basis of monopolin recruitment to the kinetochore.
Article in Chromosoma, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Using Yeast Two-Hybrid Screening and Structural Modeling to Identify Candidate Hrr25 Kinase Interactors at the Meiotic Kinetochore inInternational journal of molecular sciences · 2026Article
- Specialisation of meiotic kinetochores revealed through a synthetic spindle assembly checkpoint strategy.eLife · 2026Article
- Evolutionary cell biology comes of age.Journal of cell science · 2025Article
- The composition and structure of the outer kinetochore KMN complex is conserved across kingdoms.Communications biology · 2025Article
- Article
- A stable microtubule bundle formed through an orchestrated multistep process controls quiescence exit.eLife · 2024Article
- The Four Causes: The Functional Architecture of Centromeres and Kinetochores.Annual review of genetics · 2022Review
- Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution.Frontiers in cell and developmental biology · 2022Review
- Loss of sister kinetochore co-orientation and peri-centromeric cohesin protection after meiosis I depends on cleavage of centromeric REC8.Developmental cell · 2021Article
- The Proteomic Landscape of Centromeric Chromatin Reveals an Essential Role for the Ctf19Current biology : CB · 2021Article
- Meiosis I Kinase Regulators: Conserved Orchestrators of Reductional Chromosome Segregation.BioEssays : news and reviews in molecular, cellular and developmental biology · 2020Article
- The Structural Basis for Kinetochore Stabilization by Cnn1/CENP-T.Current biology : CB · 2020Article
- Real-time dynamics ofJournal of cell science · 2020Article
- Special issue on "recent advances in meiosis from DNA replication to chromosome segregation".Chromosoma · 2019Article
- Mosaic origin of the eukaryotic kinetochore.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Reductional Meiosis I Chromosome Segregation Is Established by Coordination of Key Meiotic Kinases.Developmental cell · 2019Article
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Authors and funding
11 authors at 5 institutions in 4 countries.
Funding
Abstract
The monopolin complex is a multifunctional molecular crosslinker, which in S. pombe binds and organises mitotic kinetochores to prevent aberrant kinetochore-microtubule interactions. In the budding yeast S. cerevisiae, whose kinetochores bind a single microtubule, the monopolin complex crosslinks and mono-orients sister kinetochores in meiosis I, enabling the biorientation and segregation of homologs. Here, we show that both the monopolin complex subunit Csm1 and its binding site on the kinetochore protein Dsn1 are broadly distributed throughout eukaryotes, suggesting a conserved role in kinetochore organisation and function. We find that budding yeast Csm1 binds two conserved motifs in Dsn1, one (termed Box 1) representing the ancestral, widely conserved monopolin binding motif and a second (termed Box 2-3) with a likely role in enforcing specificity of sister kinetochore crosslinking. We find that Box 1 and Box 2-3 bind the same conserved hydrophobic cavity on Csm1, suggesting competition or handoff between these motifs. Using structure-based mutants, we also find that both Box 1 and Box 2-3 are critical for monopolin function in meiosis. We identify two conserved serine residues in Box 2-3 that are phosphorylated in meiosis and whose mutation to aspartate stabilises Csm1-Dsn1 binding, suggesting that regulated phosphorylation of these residues may play a role in sister kinetochore crosslinking specificity. Overall, our results reveal the monopolin complex as a broadly conserved kinetochore organiser in eukaryotes, which budding yeast have co-opted to mediate sister kinetochore crosslinking through the addition of a second, regulatable monopolin binding interface.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.