ArticleVirus evolution2019
Intra- and interpatient evolution of enterovirus D68 analyzed by whole-genome deep sequencing.
Article in Virus evolution, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Progress in enterovirus D68-neutralizing antibodies and vaccines.Journal of virology · 2026Review
- A self-amplifying RNA vaccine prevents enterovirus D68 infection and disease in preclinical models.Science translational medicine · 2024Article
- Real-Time Enterovirus D68 Outbreak Detection through Hospital Surveillance of Severe Acute Respiratory Infection, Senegal, 2023.Emerging infectious diseases · 2024Article
- Changes in transmission of Enterovirus D68 (EV-D68) in England inferred from seroprevalence data.eLife · 2023Article
- Pneumococcal within-host diversity during colonization, transmission and treatment.Nature microbiology · 2022Article
- Evolution, geographic spreading, and demographic distribution of Enterovirus D68.PLoS pathogens · 2022Article
- Exploring a prolonged enterovirus C104 infection in a severely ill patient using nanopore sequencing.Virus evolution · 2022Article
- Off-season circulation and characterization of enterovirus D68 with respiratory and neurological presentation using whole-genome sequencing.Frontiers in microbiology · 2022Article
- Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Genome Sequencing from Post-Mortem Formalin-Fixed, Paraffin-Embedded Lung Tissues.The Journal of molecular diagnostics : JMD · 2021Article
- Article
- Molecular Epidemiology and Evolutionary Trajectory of Emerging Echovirus 30, Europe.Emerging infectious diseases · 2021Article
- Human antibodies neutralize enterovirus D68 and protect against infection and paralytic disease.Science immunology · 2020Article
- Linking influenza virus evolution within and between human hosts.Virus evolution · 2020Article
- Origin of HAV strains responsible for 2016-2017 outbreak among MSM: Viral phylodynamics in Lazio region.PloS one · 2020Article
- Co-circulation of multiple enterovirus D68 subclades, including a novel B3 cluster, across Europe in a season of expected low prevalence, 2019/20.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Worldwide outbreaks of enterovirus D68 (EV-D68) in 2014 and 2016 have caused serious respiratory and neurological disease. To investigate diversity, spread, and evolution of EV-D68 we performed near full-length deep sequencing in fifty-four samples obtained in Sweden during the 2014 and 2016 outbreaks. In most samples, intrapatient variability was low and dominated by rare synonymous variants, but three patients showed evidence of dual infections with distinct EV-D68 variants from the same subclade. Interpatient evolution showed a very strong temporal signal, with an evolutionary rate of 0.0039 ± 0.0001 substitutions per site and year. Phylogenetic trees reconstructed from the sequences suggest that EV-D68 was introduced into Stockholm several times during the 2016 outbreak. Putative neutralization targets in the BC and DE loops of the VP1 protein were slightly more diverse within-host and tended to undergo more frequent substitution than other genomic regions. However, evolution in these loops did not appear to have been driven the emergence of the 2016 B3-subclade directly from the 2014 B1-subclade. Instead, the most recent ancestor of both clades was dated to 2009. The study provides a comprehensive description of the intra- and interpatient evolution of EV-D68, including the first report of intrapatient diversity and dual infections. The new data along with publicly available EV-D68 sequences are included in an interactive phylodynamic analysis on nextstrain.org/enterovirus/d68 to facilitate timely EV-D68 tracking in the future.
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