ArticleGenome biology2019
CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors.
Article in Genome biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 141 citations in OpenAlex.
- Coordinating structure-guided design and cis-regulatory optimization to engineer elite herbicide-tolerance alleles in crops.Nature plants · 2026Article
- Evolutionary tuning of a biosynthetic gene cluster drives furanocoumarin accumulation and diversification.Nature communications · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- A comprehensive all-in-one CRISPR toolbox for large-scale screens in plants.The Plant cell · 2025Article
- Selecting differential splicing methods: Practical considerations for short-read RNA sequencing.F1000Research · 2025Review
- Applications of CRISPR Technologies in Forestry and Molecular Wood Biotechnology.International journal of molecular sciences · 2024Review
- Plant Functional Genomics Based on High-Throughput CRISPR Library Knockout Screening: A Perspective.Advanced genetics (Hoboken, N.J.) · 2024Article
- SCR106 splicing factor modulates abiotic stress responses by maintaining RNA splicing in rice.Journal of experimental botany · 2024Article
- Review
- The potential of genome editing to create novel alleles of resistance genes in rice.Frontiers in genome editing · 2024Review
- Synthetic directed evolution for targeted engineering of plant traits.Frontiers in plant science · 2024Review
- Expression and Functional Analysis of the Compact ThermophilicInternational journal of molecular sciences · 2023Article
- Directed evolution rice genes with randomly multiplexed sgRNAs assembly of base editors.Plant biotechnology journal · 2023Article
- Targeted mutagenesis with sequence-specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects.The plant genome · 2023Review
- Applications and Prospects of CRISPR/Cas9-Mediated Base Editing in Plant Breeding.Current issues in molecular biology · 2023Review
- A CRISPR-based lateral flow assay for plant genotyping and pathogen diagnostics.Plant biotechnology journal · 2022Article
- CRISPR/Cas tool designs for multiplex genome editing and its applications in developing biotic and abiotic stress-resistant crop plants.Molecular biology reports · 2022Review
- Genome-Wide Association Study Reveals Novel QTLs and Candidate Genes for Grain Number in Rice.International journal of molecular sciences · 2022Article
- Synthetic evolution of herbicide resistance using a T7 RNAP-based random DNA base editor.Life science alliance · 2022Article
- Turning promise into practice: Crop biotechnology for increasing genetic diversity and climate resilience.PLoS biology · 2022Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based directed evolution platform in plants to evolve the rice (Oryza sativa) SF3B1 spliceosomal protein for resistance to splicing inhibitors. SF3B1 mutant variants, termed SF3B1-GEX1A-Resistant (SGR), confer variable levels of resistance to splicing inhibitors. Studies of the structural basis of the splicing inhibitor binding to SGRs corroborate the resistance phenotype. This directed evolution platform can be used to interrogate and evolve the molecular functions of key biomolecules and to engineer crop traits for improved performance and adaptation under climate change conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.